在3D人造心脏组织上对慢性心脏收缩度调节的非临床评估
Tromondae K Feaster1, Jourdan K Ewoldt2, Anna Avila1
1Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, US Food and Drug Administration, Silver Spring, Maryland, USA.
Journal of cardiovascular electrophysiology
|March 3, 2024
概括
使用人类工程心脏组织 (ECT) 的新2天体外测定有效地模拟了慢性心脏收缩性调制 (CCM) 疗法. 这种方法通过测量改善的收缩和改变的基因表达来增强对CCM对心力衰竭影响的理解.
科学领域:
- 生物医学工程 生物医学工程
- 心血管研究研究心血管研究
- 再生医学是一种再生医学.
背景情况:
- 心脏收缩性调制 (CCM) 疗法使用电刺激来改善心力衰竭 (HF) 中的心脏功能.
- 目前对CCM机制的理解受限于缺乏人体体外试验模型.
- 开发了一种使用工程心脏组织 (ECT) 来研究CCM的新型2天体外测定.
研究的目的:
- 建立和验证用于评估CCM的慢性体外试验法.
- 评估CCM对人类心脏组织的生理和分子影响.
- 为了解心力衰竭中CCM提供非临床工具.
主要方法:
- 人类诱导的多能干细胞衍生的心肌细胞被用来创建3D电磁图.
- ECT在纤维素凝中培养心脏纤维细胞,并接受CCM或对照刺激.
- 分析了收缩性质,细胞内和心脏基因表达.
主要成果:
- 在ECT中,慢性CCM显著增加了收缩幅度和持续时间.
- 细胞内的短暂振幅被CCM增强.
- CCM改变了与心力衰竭 (NPPB) 和兴奋-收缩合 (SLC8) 相关的基因表达.
结论:
- 这项研究在3D人体ECT模型中首次进行慢性CCM评估.
- 开发的试验作为一种有价值的非临床工具,用于研究CCM对心脏电生理学的影响.
- 这种体外试验床允许详细评估CCM对人类心脏组织的生理和分子影响.
关键词:
电子电信 (ECT) 是一种电子电信.处理处理处理处理调节心脏收缩能力的调节.这是心脏微型捆绑.心脏微组织的心脏微组织.这是心肌细胞 (cardiomyocytes).这是hiPSC-CMCM.临床前研究的临床前研究.更多相关视频
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