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黑色素通过机械向拉巴胺素 (mTOR) 途径抵消诱导的老鼠丸毒性

Mariem B Rhouma¹, Massimo Venditti², Asma Haddadi¹

¹Laboratoire LR11ES41 Génétique Biodiversité et Valorisation des Bio-ressourcés, Institut Supérieur de Biotechnologie de Monastir, Université de Monastir, Monastir, Tunisia.

Journal of experimental zoology. Part A, Ecological and integrative physiology

|March 4, 2024

概括

黑色素 (MLT) 保护大鼠丸免受 (Cd) 损伤,哺乳动物目标的拉巴胺素 (mTOR) 途径起着部分作用. 仅仅抑制mTOR就会对丸功能和精子质量产生负面影响.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
分子内分泌学分子内分泌学

背景情况:

已知 (Cd) 暴露会在老鼠中诱导丸损伤.
黑素 (MLT) 已证明对Cd诱导的丸毒性有保护作用.
在MLT的保护作用的基础上,精确的分子机制仍然不清楚.

研究的目的:

研究哺乳动物目标拉巴胺素 (mTOR) 途径在调解MLT对Cd诱导的丸损伤的保护作用中的作用.
阐明MLT对丸组织中Cd毒性的抗作用的分子机制.

主要方法:

鼠被暴露在 (Cd) 中,并用美拉 (MLT) 和/或拉帕 (Rapa),一种mTOR抑制剂进行治疗.
丸形态的评估,胚胎和体细胞自,血清丸激素水平,以及类固醇生成的蛋白质表达和血液丸屏障标记.
评价精子参数和体反应能力.

主要成果:

暴露于Cd引起丸损伤,而MLT则表现出保护作用.
用Rapa抑制mTOR在暴露于Cd的老鼠中消除了MLT的保护作用,这表明mTOR途径的部分调制.
单独抑制mTOR导致丸形态的改变,自的增加,丸激素的减少,降低了类固醇生成和血液丸屏障的完整性,以及精子功能不佳.

结论:

mTOR途径部分参与调解MLT对Cd诱导的丸毒性的保护作用.
黑色素 (MLT) 可以被认为是一个潜在的补充剂,以提高丸功能和改善男性性伴侣质量在Cd暴露的背景下.
需要进一步的研究,以充分界定MLT对丸健康的作用所涉及的分子途径.

关键词:

血液试剂是屏障的障碍.和是最重要的元素.在 mTOR 路径上.黑激素是什么意思黑激素是什么意思这种药物是拉帕米辛 (Rapamycin).精子的产生是精子的产生.

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