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基因组编辑和一个Arabidopsis转录因子的分子分析,晚期开花

Yoshimi Nakano¹, Maki Kawai^1,2, Moeca Arai^1,2

¹Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.

Plant biotechnology (Tokyo, Japan)

|March 4, 2024

概括

晚期开花 (LATE) 蛋白质在Arabidopsis中充当转录抑制剂. 它与TOPLESS和CRABS CLAW (CRC) 相互作用,以调节花的发育和确定性.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
发育生物学是发展生物学.

背景情况:

花器官形成的时间对于植物的繁殖成功至关重要.
晚期开花 (LATE) 过度表达诱导晚期开花,通过抑制Arabidopsis.的T (FT) 开花位置.
LATE是一种C2H2指转录因子,具有EAR动机,表明转录抑制,但其功能尚不清楚.

研究的目的:

阐明LATE的分子功能,并确定相互作用的因素.
为了研究LATE的转录活性和蛋白质相互作用.
了解LATE在花发育和水系决定中的作用.

主要方法:

基因组编辑来创造晚期突变.
交易活化活动测试以评估LATE的压制能力.
酵母两杂交测试以确定蛋白质相互作用伙伴.

主要成果:

经过基因组编辑的晚期突变体没有表现出明显的表型,这表明功能冗余.
LATE表现出转录抑制活动,通过其EAR动机和其他序列进行介导.
LATE通过其EAR动机与转录核心压缩器TOPLESS以及通过其他序列与CRABS CLAW (CRC) 相互作用.

结论:

在Arabidopsis中,LATE作为转录抑制剂起作用.
LATE与TOPLESS和CRC相互作用,表明它参与了监管复杂.
晚可能与CRC形成一个转录抑制综合体,以调节花干系的决定性.

关键词:

C2H2 指 C2H2 指在 EAR 图案中,使用 EAR 图案.花开的时候,花开的时候.转录抑制剂转录抑制剂

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