在固态发酵下由Aspergillus sp.进行细胞酶生产. IN5:参数优化和应用程序
Antika Boondaeng1, Jureeporn Keabpimai1, Chanaporn Trakunjae1
1Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, Thailand.
Heliyon
|March 4, 2024
概括
这项研究优化了使用大豆残留物在固态发酵下使用真菌细胞酶的生产. 这些酶有效地化了性预处理的米,产生了高降解糖,用于可持续的废物管理.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 工业微生物学 工业微生物学
背景情况:
- 微生物细胞酶是具有广泛应用的关键工业催化剂.
- 细胞酶系统是复杂的,因其工业相关性而受到广泛研究.
- 农业废物对可持续废物管理构成了挑战.
研究的目的:
- 为了优化从新型真菌分离物, *Aspergillus* sp. 的细胞酶生产. IN5,使用固态发酵.
- 为了评估产生的细胞酶在化预处理的基纤维素农业废物的效率.
- 通过农业残留物的酶降解来探索可持续的废物管理策略.
主要方法:
- *Aspergillus* sp. 的固态发酵方法 关于大豆残留物的IN5.
- 优化发酵参数:基质,温度,pH值和化时间.
- 酸和预处理的米的酶性水解,使用产生的细胞酶.
主要成果:
- 优化的细胞酶生产在35°C,pH7.0和5天内实现了最高的总细胞酶活性 (0.26U/g),碳氧甲基细胞酶 (3.32U/g) 和β-葡萄糖酶 (196.09U/g).
- 与酸性预处理相比,米的性预处理导致了较高的降糖产量.
- 在24小时的化后,使用性预处理的米,以15U/g的酶负荷达到286.06 mg/g的最大降糖产量.
结论:
- 这项研究成功地优化了来自*Aspergillus* sp.的细胞酶生产. 在IN5.5中,我们可以使用IN5.
- 产生的真菌细胞酶有效降解纤维纤维素材料,特别是先处理的米.
- 这项研究为农业废物利用和酶生物质转化提供了可持续的方法.
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