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相关概念视频

Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
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Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
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Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
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Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

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相关实验视频

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Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles
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在固态发酵下由Aspergillus sp.进行细胞酶生产. IN5:参数优化和应用程序

Antika Boondaeng1, Jureeporn Keabpimai1, Chanaporn Trakunjae1

  • 1Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, Thailand.

Heliyon
|March 4, 2024
PubMed
概括

这项研究优化了使用大豆残留物在固态发酵下使用真菌细胞酶的生产. 这些酶有效地化了性预处理的米,产生了高降解糖,用于可持续的废物管理.

关键词:
细胞酶生成的真菌产生细胞质.菌细胞酶是一种真菌细胞酶.优化优化 优化优化米草是一个很好的方法.固态发酵发酵的固态发酵方式

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科学领域:

  • 生物技术是生物技术.
  • 酶学 是一种酶学.
  • 工业微生物学 工业微生物学

背景情况:

  • 微生物细胞酶是具有广泛应用的关键工业催化剂.
  • 细胞酶系统是复杂的,因其工业相关性而受到广泛研究.
  • 农业废物对可持续废物管理构成了挑战.

研究的目的:

  • 为了优化从新型真菌分离物, *Aspergillus* sp. 的细胞酶生产. IN5,使用固态发酵.
  • 为了评估产生的细胞酶在化预处理的基纤维素农业废物的效率.
  • 通过农业残留物的酶降解来探索可持续的废物管理策略.

主要方法:

  • *Aspergillus* sp. 的固态发酵方法 关于大豆残留物的IN5.
  • 优化发酵参数:基质,温度,pH值和化时间.
  • 酸和预处理的米的酶性水解,使用产生的细胞酶.

主要成果:

  • 优化的细胞酶生产在35°C,pH7.0和5天内实现了最高的总细胞酶活性 (0.26U/g),碳氧甲基细胞酶 (3.32U/g) 和β-葡萄糖酶 (196.09U/g).
  • 与酸性预处理相比,米的性预处理导致了较高的降糖产量.
  • 在24小时的化后,使用性预处理的米,以15U/g的酶负荷达到286.06 mg/g的最大降糖产量.

结论:

  • 这项研究成功地优化了来自*Aspergillus* sp.的细胞酶生产. 在IN5.5中,我们可以使用IN5.
  • 产生的真菌细胞酶有效降解纤维纤维素材料,特别是先处理的米.
  • 这项研究为农业废物利用和酶生物质转化提供了可持续的方法.