抗生素和扩展光谱β-乳糖酶产生病原体的分子特征,涉及慢性支性中耳炎
Francis Amadi Ibiam1, Eze Egwu2, Ikechukwu Benjamin Moses3
1Department of Otorhinolaryngology, Alex Ekwueme Federal University, Ndufu Alike Ikwo, Nigeria.
The Pan African medical journal
|March 4, 2024
概括
扩展谱β-乳糖酶 (ESBL) 生产细菌在慢性性中耳炎 (CSOM) 中很常见. 对这些抗生素耐药性病原体的早期查对于有效治疗和控制抗菌素耐药性传播至关重要.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 产生β-乳糖酶的细菌,特别是扩展光谱β-乳糖酶 (ESBL) 生产者,具有临床意义,并且与慢性中耳炎 (CSOM) 治疗失败有关.
- 了解CSOM中这些耐药病原体的流行率和特征对于有效的患者管理至关重要.
研究的目的:
- 确定从CSOM患者中分离的ESBL产生格兰阴性细菌 (GNB) 病原体的频率,抗微生物敏感性模式 (抗生素) 和分子特征.
主要方法:
- 标准的微生物学技术被用来分析来自CSOM患者的300个耳部拭子样本.
- 通过Kirby-Bauer磁盘扩散来确定抗菌素敏感性.
- 使用双盘协同测试 (DDST) 和聚合酶链反应 (PCR) 进行了ESBL产生的GNB的表型检测和分子特征.
主要成果:
- 大肠杆菌和P. aeruginosa在CSOM患者中普遍存在,排放持续时间超过两周.
- 在GNB病原体中,ESBL生产者的频率为18.3%.
- 虽然通常具有多药耐药性,但分离物对西普罗夫洛克萨,伊米佩内姆和阿米卡具有很高的敏感性. blaSHV基因 (47.6%) 是PCR检测到的最主要的ESBL基因型,其次是blaTEM (25.2%) 和blaCTX-M (10.7%).
结论:
- 这项研究强调了在CSOM感染中产生β-乳糖酶的细菌的发生和传播.
- 在CSOM中早期查抗生素耐药性病原体对于适当的抗菌疗法至关重要.
- 实施此类查措施可以帮助制抗菌素耐药性的日益蔓延.
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