使用多分形形式主义评估生物组织形态学的实用方面
A M Ignatova1, M A Zemlyanova2, O B Naimark3
1Senior Researcher; Institute of Continuous Media Mechanics of the Ural Branch of the Russian Academy of Sciences - а Branch of the Perm Federal Research Center, Ural Branch of the Russian Academy of Sciences, 13A Lenina St., Perm, 614990, Russia; Researcher; Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, 82 Monastyrskaya St., Perm, 614045, Russia.
Sovremennye tekhnologii v meditsine
|March 4, 2024
概括
多分体分析显示,在Wistar大鼠中,肺组织形态有明显的差异. 病理变化简化了气泡膜模式,改变了多分体光谱,特别是在更高的放大度.
科学领域:
- 数字病理学数字病理学
- 生物物理学的生物物理.
- 形态测量法 形态测量法 形态测量法
背景情况:
- 评估生物组织形态对于理解生理和病理过程至关重要.
- 传统方法可能缺乏捕捉复杂结构变化的灵敏度.
- 多分法分析提供了一种定量方法来表征复杂的模式.
研究的目的:
- 探索多分形形式主义在生物组织形态学评估中的实际应用.
- 使用多分体特性,区分健康和病态改变的肺组织.
主要方法:
- 分析了Wistar大鼠肺组织 (健康和病理) 的组织图像.
- 使用ImageJ/Fiji与FracLac模块进行了图像处理和多片谱计算.
- 分析涉及确定各种放大 (50×,100×,200×) 的缩放指数和奇点光谱.
主要成果:
- 健康的肺组织表现出多重折射性,其特点是特定的奇点光谱.
- 病理组织显示左移光谱,表明膜模式的"简化".
- 较低的放大 (50×,100×) 导致病理组织中多重折射性标志的丧失.
结论:
- 对组织学图像进行准确的多分片光谱分析需要200倍放大和线性轮预处理.
- 健康和病态肺组织之间的显著形态差异可以通过光谱高度,宽度和位置来检测.
- 多分形形式主义为量化组织微观结构变化提供了一个敏感的工具.
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