基于肠道微生物群的加工Evodiae fructus的毒性评估
Xuejuan Liang1, Jing Liu2, Jiaxin Di2
1Hunan Academy of Chinese Medicine, Changsha, China.
Frontiers in microbiology
|March 4, 2024
概括
处理Evodiae fructus (EF) 改变了它对小鼠健康的影响,降低了毒性,但增加了有害的肠道细菌. 需要进一步的研究来了解临床剂量的EF肝毒性.
科学领域:
- 药理学 药理学是指药理学的学科.
- 毒理学 毒理学 毒理学
- 微生物学 微生物学
背景情况:
- 药物的有效性和安全性在医疗保健中至关重要.
- 处理像Evodiae fructus (EF) 这样的药草可以改变它们的毒性和有效性.
- 了解加工对EF的影响对于安全的临床应用至关重要.
研究的目的:
- 研究加工对Evodiae fructus (EF) 的毒性和肠道微生物群的影响.
- 在小鼠模型中比较原始和加工EF的毒理学概况.
主要方法:
- 50只雄性昆明小鼠被分为对照组和EF治疗组 (原料和加工,小和中等花型EF).
- 小鼠每天接受EF提取物21天以5倍的药物库剂量.
- 评估肝/功能 (AST,ALT,SCr,BUN),进行组织病理学,并通过16SrRNA测序分析肠道微生物群.
主要成果:
- 服用EF导致体重减轻,/肝酶水平增加.
- 与原始EF (CRMEF) 相比,加工EF (CPMEF) 的毒性降低.
- CPMEF显著改变了肠道微生物群,增加了有害的细菌 (葡萄球菌,叶菌,盐球菌),减少了有益的细菌.
结论:
- 加工Evodiae fructus可以降低它在小鼠中的毒性.
- 经过加工的EF显著改变了肠道微生物组的组成.
- 在测试剂量下,EF肝毒性并不显著,可能是由于度和持续时间.
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