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VvATG6通过增强转基因葡萄中的抗氧化能力,有助于提高铜的应激耐受性

Jiaxin Xia^1,2, Zicheng Wang^1,2,3, Siyu Liu^1,2

¹Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095 China.

Physiology and molecular biology of plants : an international journal of functional plant biology

|March 4, 2024

概括

过度表达葡萄酒ATG6 (VvATG6) 通过提高抗氧化活性和平衡活性氧物种,提高植物对铜应激的耐受性. 这项研究揭示了VvATG6.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

在非生物压力期间,自对于植物细胞平衡至关重要.
与自相关的基因 (ATG) 参与了应激反应,但它们在铜 (Cu) 应激耐受性中的作用尚未得到充分研究.
了解特定的ATG功能对于开发抗压作物至关重要.

研究的目的:

克隆和表征一种新的葡萄酒ATG6基因 (VvATG6).
调查VvATG6在植物中对铜应激的耐受性赋予作用.
阐明VvATG6-介导的铜耐受性背后的分子机制.

主要方法:

从葡萄酒植物中克隆VvATG6.
通过Agrobacterium介导的转化产生转基因的Arabidopsis thaliana,Nicotiana tabacum和葡萄.
分析植物生长,生物质,抗氧化酶活动,活性氧物种 (ROS) 水平和铜应力下的基因表达.

主要成果:

铜应力诱导了VvATG6的表达.
过度表达VvATG6增强了对铜的耐受性,从而改善了转基因植物的生长和生物量.
转基因植物表现出增加的抗氧化酶活性 (SOD,POD,CAT),降低了甲和过氧化的积累,以及ROS相关基因 (RbohB,RbohC) 的调节表达,表明ROS平衡的稳态.

结论:

VvATG6在增强植物对铜压力的耐受性方面发挥着重要的保护作用.
VvATG6介导的耐受性涉及抗氧化剂防御系统的上调和ROS生产和清理的调节.
这项研究提供了第一个证据,证明VvATG6在植物对铜的耐受性方面的功能,为改善作物提供了潜在的可能性.

关键词:

自自是一种自的过程.在压力 Cu 压力 Cu 压力.ROS的合成和清理.转基因葡萄卡利 (calli calli) 是一种转基因葡萄.在VvATG6中使用VvATG6.

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