保护性自增强通过AMPK/ULK1信号通路的人类永生化角质细胞的抗压能力
Zhinan Shi1,2, Jing Wang1,2, Min Li3
1Department of Dermatology, Nantong Third People's Hospital, Affiliated Nantong Hospital 3 of Nantong University, Nantong, China.
Cell biology international
|March 4, 2024
概括
低剂量5-氨基烯酸光动力疗法 (ALA-PDT) 通过调节AMPK/ULK1通路,激活了角质细胞的自细胞. 这样可以增强皮肤的颜色.
科学领域:
- 皮肤病学和细胞生物学
- 光动力学治疗机制 光动力学治疗机制
背景情况:
- 角质细胞形成皮肤的外部屏障,对抵抗环境损伤至关重要.
- 细胞自对于维持状细胞的平衡和去除损坏的部件至关重要.
- 精确的5 - 氨基烯酸光动力学疗法 (ALA-PDT) 调节质细胞自的机制尚未完全理解.
研究的目的:
- 在低剂量ALA-PDT后,研究人类角质细胞 (HaCaT细胞) 中自的调节机制.
- 为了确定关键的分子通路和参与ALA-PDT诱导自的蛋白质.
- 为了确定ALA-PDT介导的自是否有助于增强皮肤细胞的抗压力.
主要方法:
- 用低剂量的ALA-PDT (0.5 mmol/L,3 J/cm2) 治疗HaCaT细胞.
- 用RNA测序来分析与自相关的全球基因表达变化.
- 西方涂抹被用于量化关键的自相关蛋白质 (Beclin-1,LC3-II/LC3-I).
- 评估了细胞参数,包括活性氧物种 (ROS) 水平,线粒体膜潜力和ATP生产.
- 研究了AMP激活蛋白激酶 (AMPK) /Unc-51样蛋白激酶1 (ULK1) 途径.
- 紫外线B (UVB) 辐射被用于模拟环境压力,并测量了细胞亡与或没有自抑制剂 (3-甲基氨酸).
主要成果:
- 低剂量的ALA-PDT调节了与自相关的途径,并将Unc-51样酶1 (ULK1) 确定为关键基因.
- ALA-PDT提高了Beclin-1的调节,并增加了LC3-II/LC3-I比率,表明了自细胞的激活.
- 自通过AMPK/ULK1通路被激活,涉及ROS诱导,短暂的线粒体潜能减少和ATP产生减少.
- ALA-PDT 预处理减轻了 HaCaT 细胞中的 UVB 诱导的亡.
- ALA-PDT对UVB损伤的保护作用被自抑制剂3-甲基氨酸取消.
结论:
- 低剂量的ALA-PDT通过ROS依赖的AMPK/ULK1信号通路激活了角质细胞中的细胞自.
- 这种由ALA-PDT诱导的自增强了角质细胞的抗压力能力,提供了对UVB辐射等环境损害的保护.
- 通过低剂量的ALA-PDT准自是一种潜在的策略,可以提高皮肤对环境压力因素的抵抗力.
相关概念视频
Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
PI3K/mTOR/AKT Signaling Pathway
3.6K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.6K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K
MAPK Signaling Cascades
5.5K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.5K
Delivery Pathways to the Lysosome
6.5K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.5K


