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相关概念视频

Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

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The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
7.1K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

7.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
7.0K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.5K
Transport Across the Golgi01:26

Transport Across the Golgi

4.2K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
4.2K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

11.1K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
11.1K

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相关实验视频

Updated: Jul 1, 2025

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
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Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

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性诱导的货物加载到细胞外囊中.

Chaeeun Lee1,2, Sumit Kumar1,2, Juhee Park2

  • 1Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea. ykcho@unist.ac.kr.

Lab on a chip
|March 4, 2024
PubMed
概括

这项研究引入了强度控制 (TC),一种用于将药物加载到细胞外囊泡 (EVs) 的新方法. TC提高了药物加载效率,并保持了潜在治疗应用的EV完整性.

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Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons
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Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis
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相关实验视频

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Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis
11:09

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科学领域:

  • 生物技术是生物技术.
  • 纳米技术纳米技术
  • 药物输送系统 药物输送系统

背景情况:

  • 细胞外囊泡 (EVs) 显示出作为药物输送载体的前景.
  • 控制EV膜的透性,以有效地加载货物仍然是一个挑战.
  • 在加载期间保持EV完整性和功能对于治疗疗效至关重要.

研究的目的:

  • 开发一种快速,高效和温和的方法来将分子装入电动汽车.
  • 使用调度控制 (TC) 精确控制 EV 膜的透性.
  • 评估TC方法与传统装载技术相比的有效性.

主要方法:

  • 实验室在磁盘平台被用于调度控制 (TC) 方法.
  • 电动汽车使用低压溶液暂时透,然后进行同位素冲洗.
  • 将多克索鲁比辛化 (Dox),ssDNA和miRNA加载到EV中使用TC方法进行.

主要成果:

  • 与超声波 (4.3倍) 和挤出 (7.2倍) 相比,TC方法显示了显著更高的负载产量.
  • 通过TC加载,维护了电动汽车的完整性和功能.
  • 细胞内评估证实了TC制备的miRNA-497载荷EV和多克索鲁比载荷EV的优异性能.

结论:

  • 调度控制 (TC) 提供了一种有效和温和的方法,用于将各种货物装入细胞外囊泡 (EVs).
  • 这种TC方法提高了加载效率,并保持了EV完整性,为基于外体细胞的先进疗法铺平了道路.
  • 封装效率显著影响治疗结果,突出了TC在临床应用中的潜力.