用酶和pH响应度调节水性聚合物的抗菌活性
Desoshree Ghosh1, Sandeep Yadav2, Sagar Bag1
1Polymer Research Centre and Centre for Advanced Functional Materials, Department of Chemical Sciences, Indian Institute of Science Education and Research Kolkata, Mohanpur, Nadia, West Bengal 741246, India. p_de@iiserkol.ac.in.
Journal of materials chemistry. B
|March 4, 2024
概括
新的抗菌共聚合物使用可逆添加-碎片化链转移 (RAFT) 聚合而开发. 这些聚合物形成具有强大的杀菌活性和高生物安全性的纳米聚合物,用于向药物输送.
科学领域:
- 聚合物化学 聚合物化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 细胞膜脂质组成在原核生物和真核生物之间有所不同,这使得向治疗变得复杂.
- 开发有效的抗菌剂需要平衡疏水性/疏水性,并确保生物安全.
- 酶和pH响应药物递送系统提供了增强的治疗潜力.
研究的目的:
- 合成具有集成的疏水/疏水平衡,阴离子电荷和酶/pH响应性的新型交替共聚物.
- 描述这些共聚合物的自我组装成纳米聚合物,以实现潜在的治疗应用.
- 评估开发的聚合物纳米聚合物的抗菌疗效和生物安全性.
主要方法:
- 可逆添加-碎片化链转移 (RAFT) 聚合被用来合成氨酸附着的氨酸和脂肪酸结合的马莱胺单体.
- 动态光散射 (DLS) 和泽塔电位测量描述了纳米聚合物形成和表面电荷.
- 尼罗河红色染料的体外释放动力学,汁微稀释试验,FESEM和细胞活力试验评估了药物释放,抗菌活性和生物安全性.
主要成果:
- 合成的聚合物形成了稳定的纳米聚合物 (30-40纳米),具有正的泽塔潜力.
- 纳米聚合物显示pH/酶触发的释放封装的疏水性染料 (约. 在pH 5.5下85%的释放与脂酶).
- 观察到针对格拉姆阳性和格拉姆阴性细菌 (MIC50 < 30μg mL-1) 的显著杀菌活性,具有较低的溶血活性和对人类肠道细胞的最小毒性.
结论:
- 开发的聚合物纳米聚合物具有可调节的疏水/疏水性质以及对pH和酶的反应性.
- 这些纳米聚合物表现出强大的广泛抗菌活性.
- 它们的高生物安全性说明了它们作为抗菌剂和药物输送系统的体内应用的潜力.
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