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Updated: Jul 1, 2025

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

在Fusobacterium nucleatum中进行的转录组精细映射揭示了FoxJ,这是一个新的s^E依赖的小RNA,具有不寻常的mRNA激活活动

Falk Ponath¹, Yan Zhu¹, Jörg Vogel^1,2

¹Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany.

|March 4, 2024

概括

这项研究确定了FoxJ,这是Fusobacterium核中的一种新的小RNA (sRNA),有助于调节压力反应和基因表达. 与FoxI一起,FoxJ控制FomA蛋白,而FoxJ独特地激活其他基因,扩大了我们对细菌适应性的理解.

科学领域:

微生物学微生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

Fusobacterium nucleatum是一种口腔细菌,与癌症和早产等疾病有关.
它独特的进化位置阻碍了对其适应性RNA调节网络的理解.
之前发现的一种全球应激反应涉及西格玛因子 σE 和一个名为FoxI的小RNA (sRNA).

研究的目的:

在F. nucleatum.中发现新的 σE 依赖小RNA (sRNA).
为了全面地绘制F. nucleatum的转录起点和停止点.
了解 σE应激反应中新发现的sRNAs的调节作用.

主要方法:

在F. nucleatum中全球转录末端映射 (5'和3'末端) ATCC 23726.6.
新型小RNAs (sRNAs) 的识别和表征.
对sRNA调节基因表达的分析,包括mRNA抑制和激活.

主要成果:

发现了FoxJ,一个~156核酸sRNA,以前被误解.
FoxJ受 σE 的控制,在压力条件下被激活,类似于 FoxI.
福克斯I和福克斯J都抑制FomA蛋白合成;福克斯J独特地调节额外的基因并通过针对终端器的新型机制激活基因表达.

结论:

这项研究提供了F. nucleatum.中5'和3'UTR的全面注释.
FoxJ 是 σE 应激反应的新型,保存的组成部分,扩展已知的调节机制.
这些发现揭示了sRNA介导的mRNA激活的新模式,为细菌适应提供了见解.

关键词:

在RNA-seqqq.包裹压力压力是什么超细胞质的西格玛因子.没有编码的RNA.转录后的控制转录后的控制小小的RNARNA小小的RNARNA.

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