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相关概念视频

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jul 1, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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一个开源的,高分辨率的,自动化的光显微镜.

Ando Christian Zehrer1, Ana Martin-Villalba2, Benedict Diederich3

  • 1Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.

eLife
|March 4, 2024
PubMed
概括
此摘要是机器生成的。

研究人员使用开源硬件和软件开发了一种负担得起的高分辨率光显微镜. 这种低预算的系统可以为实验室和教育目的提供高质量的成像,实现单分子灵敏度.

关键词:
哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈细胞生物学 细胞生物学细胞培养培养的细胞培养.人类 人类 人类 人类 人类 人类 人类活细胞是一种活细胞.

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科学领域:

  • 生物物理学的生物物理.
  • 显微镜的使用方法
  • 开源的硬件是开源的.

背景情况:

  • 复杂的光显微镜对于生命科学是必不可少的,但价格昂贵,在数据生产中造成瓶.
  • 开源硬件,利用3D打印和低成本电子产品,提供了一个潜在的解决方案.
  • 现有的低成本选择往往会损害图像质量,而先进的设计需要大量的专业知识.

研究的目的:

  • 使用开源组件和高质量部件组装一个自动化的高分辨率光显微镜.
  • 为了证明系统对固定和活样本的高分辨率成像的能力.
  • 为了验证系统的长期活细胞成像,单分子灵敏度和超分辨率显微镜的性能.

主要方法:

  • 集成UC2显微镜工具箱与高质量的组件,电子设备和软件.
  • 一个自动化,高分辨率的光显微镜的组装.
  • 使用固定和活生物样本进行测试,包括长期化和超分辨率实验.

主要成果:

  • 成功组装了一种自动化,高分辨率的光显微镜.
  • 对固定和活样本进行高分辨率成像的演示,包括多天活细胞成像.
  • 获得单分子灵敏度,使单个粒子跟踪和SMLM超分辨率显微镜成为可能.

结论:

  • 低预算的开源光显微镜可以实现高图像质量和与商业系统相比的高级功能.
  • 该系统赋予研究人员有限的资源,并作为有价值的教育材料.
  • 高质量的科学数据生成是可行的,对于用户使用的技术专业知识是最小的,使用负担得起的,开源工具.