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在细胞内注射精子 (ICSI) 穿孔膜后,zwitterionic缓冲体的流入会改变人类卵细胞的转录组

Robert J Mendola¹, Leelabati Biswas^2,3, Karen Schindler²

¹Institute for Reproductive Medicine and Science (IRMS) at Saint Barnabas, Livingston, NJ, USA. Bmendy01@msn.com.

Journal of assisted reproduction and genetics

|March 4, 2024

概括

在ICSI期间穿透卵细胞膜允许缓冲流入,改变基因表达,并可能影响胚胎发育. 双碳酸缓冲剂可能为改善IVF结果提供比HEPES和MOPS更安全的替代品.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
基因组学就是基因组学.

背景情况:

内细胞质精子注射 (ICSI) 涉及穿透卵细胞膜.
周围缓冲流对卵细胞质量和发育能力的潜在影响尚未完全理解.

研究的目的:

调查ICSI期间穿透卵细胞膜是否允许缓冲流入人类卵细胞.
确定对卵细胞发育能力和转录基因组的影响.

主要方法:

使用共聚焦光显微镜,评估膜穿孔后80个人类MII卵子细胞的缓冲流入.
超低输入RNA测序分析了40个暴露在HEPES,MOPS或二碳酸盐缓冲器中的人类MII卵细胞的转录组.

主要成果:

穿透卵细胞膜导致了不受限制的缓冲流入,超出了程序控制.
转录组分析显示,细胞骨转录减少,并改变了胚胎能力和与压力相关的转录,这取决于所使用的缓冲区.
HEPES和MOPS缓冲剂与增加的压力标志物有关,而二碳酸盐显示出更高的胚胎能力转录.

结论:

菌的缓冲剂可能会对卵细胞内的内在细胞机制产生负面影响.
在ICSI期间HEPES和MOPS缓冲器的涌入可能会造成压力,可能会阻碍胚胎发育.
建议在ICSI期间使用二碳酸盐缓冲剂作为首选的卵细胞持有介质,以减轻压力并改善试管婴儿的结果.

关键词:

二碳酸盐缓冲器 ICSI 的希佩斯 (Hepes) 是一个希佩斯.美国国务院调查局 (ICSI)MOPS MOPS MOPS 的意思是卵细胞转录组的转录组兹维特里奥尼克缓冲器

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