极端重组控制深度保护植物干细胞调节器的 cis 监管区域
Danielle Ciren1, Sophia Zebell2,3, Zachary B Lippman1,2,3
1Cold Spring Harbor Laboratory, School of Biological Sciences, Cold Spring Harbor, New York, United States of America.
PLoS genetics
|March 4, 2024
概括
保存的植物基因显示出截然不同的调节DNA,即使功能被保留. 这项研究揭示了 cis-regulatory 进化如何影响跨物种的基因功能和特征变异.
科学领域:
- 进化遗传学的进化遗传学
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
背景情况:
- 保存的基因往往在物种之间具有不同的cis-regulatory序列.
- 尽管存在 cis 调节进化,保留基因功能的机制仍不清楚.
- 在物种内部对表型变化的cis-regulatory变化的影响尚未完全理解.
研究的目的:
- 研究一种深度保存的植物干细胞调节器中的cis调节进化.
- 确定 cis-调节序列差异如何影响基因功能和表型结果.
- 探索 cis 调节变异对基因型与表型关系的影响.
主要方法:
- 在Arabidopsis thaliana和Solanum lycopersicum中使用了体内基因组编辑.
- 在CLAVATA3 (CLV3) 基因的cis-regulatory区域产生了超过70个缺失等位基因.
- 评估了这些删除对卡佩尔数的影响,这是一个共享的表型特征.
主要成果:
- 番茄CLV3上游序列对干扰很敏感,而阿拉比多普西斯CLV3更耐受.
- 在阿拉比多普西斯中,联合的 cis-regulatory 删除显示出协同效应,与番茄中的添加效应不同.
- 在物种之间证明了功能性cis-regulatory序列的明显分布和冗余性.
结论:
- 保存基因的Cis调节序列表现出了显著的进化形性.
- cis调节序列的重新配置是影响基因型与表型关系的显著进化力量.
- 对cis-regulatory架构的谱系特定分析对于作物的工程特征至关重要.
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