由远距离相关的F等离子体之间交换的Tra子单元组成的化学系统揭示了IV型分泌机器之间惊人的可塑性
Kouhei Kishida1, Yang Grace Li1, Natsumi Ogawa-Kishida1
1Department of Microbiology and Molecular Genetics, McGovern Medical School at UTHealth, Houston, Texas, United States of America.
PLoS genetics
|March 4, 2024
概括
细菌类型IV分泌系统 (T4SSs) 显示出显著的可塑性,允许仿制系统形成功能性DNA转移机器. 这种灵活性解释了它们的进化多样化和移动遗传元素的传播.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 细菌IV型分泌系统 (T4SS) 对DNA和蛋白质转移至关重要,但它们的基质识别和细胞相互作用尚不清楚.
- 尽管保留了元素,但T4SS在组成和结构上表现出显著的多样性.
研究的目的:
- 调查T4SS功能多功能性的结构基础.
- 探索T4SS如何识别基质并与细胞相互作用.
主要方法:
- 在IncF等离子体pED208和F.之间进行系统的删除和子单位交换.
- 第四类合蛋白 (T4CPs) 的突变分析.
- 在NCBI数据库中对肠道细菌菌株的生物信息分析.
主要成果:
- 化学T4SSs形成了功能性DNA转移机器,表明了其内在的灵活性.
- 交换T4CPs (TraD) 在基质-TraD和TraD-T4SS接口上表现出宽松的特异性.
- 当某些组件被交换时,即使没有培根生产,DNA转移也发生了.
- 许多肠细菌菌株含有多个F型等离子体,有些缺乏自我转移组件.
- 宿主细胞中的共居等离子体形成了功能性仿真T4SS,使多个等离子体的转移成为可能.
结论:
- 由于T4SS的可塑性,可以更轻松地组装功能化马.
- 结构灵活性有助于T4SSs的进化多样化.
- 这种可塑性有助于转移缺陷的移动遗传元素的扩散.
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