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增加了Serratia marcescens的蛋白分解活性临床分离物HU1848与更高的eepR表达相关

Karla L De Anda-Mora¹, Faviola Tavares-Carreón², Carlos Alvarez¹

¹1Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico.

Polish journal of microbiology

|March 4, 2024

概括

这项研究表明,CpxR调节器在Serratia marcescens中负面控制了eepR表达. 在HU1848隔离物中,较高的eepR转录与蛋白质分解活性和毒性因子的增加有关.

科学领域:

微生物学微生物学
分子生物学分子生物学
细菌病原体的产生

背景情况:

塞拉蒂亚马尔塞森斯是一种机会性病原体,具有可变的蛋白质分解能力.
金属蛋白酶谢拉素 (PrtS) 是一个关键的毒性因子.
了解毒性因子的调节对于对抗S. marcescens感染至关重要.

研究的目的:

在S. marcescens分离物中表征蛋白酶生产和转录调节剂.
研究CpxR在调节eepR表达中的作用.
为了将蛋白酶活性与转录调节器表达相关联.

主要方法:

细胞图和质谱测量用于识别蛋白酶.
亚佐素降解和qRT-PCR测量蛋白质分解活性和基因表达.
在体内转录试验和电泳运动移位试验 (EMSA) 来研究基因调节.

主要成果:

与SmUNAM836和Db10.0相比,HU1848隔离物显示出更高的蛋白质分解活性和prtS表达.
在HU1848中观察到更高的eepR表达,由CpxR.负面调节.
CpxR直接与eepR调节区域结合,这表明在S. marcescens物种中保持了调节.

结论:

CpxR是S. marcescens中eepR的一个新型负调节器.
增加eepR转录有助于HU1848分离中的蛋白质分解活性升高.
这项研究增强了对S. marcescens分离物中的毒性因子变异性的理解.

关键词:

CpxRR 在线播放现在是EepRR.塞拉蒂亚 (Serratia marcescensens) 是一种的植物.蛋白质酶蛋白质酶是一种蛋白质.谢拉利辛是什么谢拉利辛是什么

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