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在列上的毛细血管抑制器用于开放的管状离子色谱

Weixiong Huang¹, Ge He²

¹Hubei Key Laboratory of Yangtze Catchment Environmental Aquatic Science, School of Environmental Studies, China University of Geosciences, Wuhan, 430078, Hubei, China; Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, 76019-0065, United States.

Analytica chimica acta

|March 4, 2024

概括

开发了用于抑制开放管状离子染色学 (SOTIC) 的新的柱上抑制剂. 设计C,一个柱上化学抑制器,提供有效的分析剂抑制与最小的死体积和没有分析剂损失,使其适合低流速.

科学领域:

分析化学分析化学
染色体学染色体学是一种染色学.

背景情况:

抑制剂对于抑制离子染色学,特别是抑制开放管状离子染色学 (SOTIC) 是至关重要的.
降低抑制器和连接器的死体积至关重要,以减少在低流速 (20-200nL/分钟) 运行的SOTIC系统中的峰值分散.

研究的目的:

设计和评估用于SOTIC系统的新型柱上抑制器.
为了应对死体积的挑战,并分析现有抑制器技术中的损失.

主要方法:

开发了三种柱式抑制器设计:两种电透析 (A和B设计) 和一种化学 (C设计).
设计涉及修改环烯聚合物 (COP) 和聚乙基 (PEEK) 毛细血管.
电透析抑制器在同流和反流模式下进行了测试; 化学抑制器 (设计C) 评估了效率和分析物回收.

主要成果:

电透析柱上抑制器 (A和B设计) 由于氧化物化剂抑制所需的高电压,造成了显著的分析物损失 (>90%) 和高噪声.
在同流模式下的电透析抑制器显示出作为分析剂丰富毛细血管的潜力.
列上的化学抑制剂 (C型) 证明了有效地抑制低氧化物化剂,最小的死体积 (0.27nL) 并且没有分析物损失.
设计C实现了高柱效率 (Cl-) 47000张/m,效率损失最小 (≤3%).

结论:

电透析柱上抑制器 (A和B设计) 由于分析器损失和运行问题,对于SOTIC来说并不实用.
柱上化学抑制器 (设计C) 是SOTIC的可行解决方案,提供优良的抑制和对柱效率的影响最小.
在列上的化学抑制有效地减少了死体积,这对于高性能SOTIC至关重要.

关键词:

化学抑制剂是一种化学抑制剂.同时流的同时流.反流的逆流的逆流的循环olefin聚合物中的一个.在列上抑制压抑.聚乙烯基聚乙烯基

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