基于基因组学分析了四种来自猪的乳酸细菌菌株,并评估它们作为潜在的益生菌
Dekai Niu1, Ni Feng1, Siteng Xi1
1Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing, 210095, China.
Molecular genetics and genomics : MGG
|March 4, 2024
概括
基因组分析和体外试验揭示了Lactobacillus johnsonii L16作为一个有前途的猪益生菌菌株. 这种乳酸细菌菌株表现出优越的碳水化合物利用,耐酸和胆汁耐受性,以及抗氧化和抗菌活性.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 基因组学就是基因组学.
背景情况:
- 来自猪的乳酸细菌 (LAB) 显示出作为牲畜益生菌的潜力.
- 对来自猪的LAB益生菌的基因组评估尚未得到充分研究.
- 益生菌对于牲畜健康和生产力至关重要.
研究的目的:
- 通过基因组学和体外测试来评估四种来自猪的LAB菌株的益生菌潜力.
- 通过全面的分析来确定猪生产中优秀的益生菌候选者.
- 探索选择的LAB菌株中益生菌特征的遗传基础.
主要方法:
- 基因组分析包括泛基因组和对线性评估.
- 碳水化合物活性酶 (CAE) 和KEGG通路分析.
- 在体外测试酸耐受性,胆盐耐受性,抗氧化活性和抗菌功效.
主要成果:
- 牛乳杆菌约翰尼L16表现出更高的CAE数量和更高的碳水化合物利用率.
- 这四种菌株都能耐受低pH和0.1%的胆盐,其中L. johnsonii L16具有最大的酸性耐受性.
- 在体外证实了抗氧化和抗菌活性;L. johnsonii L16和L. salivarius ZSA5具有细菌素基因集群.
结论:
- 基因组分析与体外试验相结合,是评估益生菌菌株的有效策略.
- 牛乳杆菌 johnsonii L16是猪生产的强有力的益生菌候选人,因为它具有多方面的有益特征.
- 这项研究为在猪养殖中开发新型益生菌提供了基础.
相关概念视频
01:29Modern Molecular Taxonomy
836
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
01:27Microbes in the Production of Fermented Foods
327
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
327
01:22Probiotics
296
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
296
01:25Production of Organic Acids
105
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
105