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在印度尼西亚2022年爆发的重组LSDV分离物的分子特征通过家族遗传网络和基于SNP的全基因组分析

Indrawati Sendow¹, Irene Kasindi Meki², Ni Luh Putu Indi Dharmayanti¹

¹Research Center for Veterinary Science, Research Organization for Health, National Research and Innovation Agency, West Java, Indonesia.

|March 4, 2024

概括

印尼的块性皮肤病 (LSD) 是由重组LSD病毒引起的. 分子分析显示它与其他亚洲重组剂有关,有助于控制策略.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
疾病监测疾病监测

背景情况:

结节性皮肤病 (LSD) 是一种具有重大跨境牛和水牛病毒性疾病,具有重大经济影响.
从非洲开始,LSDV已经在全球范围内传播,因此需要持续的分子监测.
在亚洲出现重组LSDV菌株的出现凸显了了解病毒演变和传播的必要性.

研究的目的:

调查2022年印度尼西亚第一个LSD疫情.
为了分子表征印尼LSDV隔离物.
将印尼分离物与其他全球LSDV菌株进行比较,并探索新的分析方法.

主要方法:

使用特定的LSDV标记基因 (RPO30,GPCR,EEV糖蛋白,B22R) 的分子表征.
印度尼西亚LSDV分离体的全基因组分析.
使用替代分析工具对分离物的比较分析和聚类,而不仅仅是全基因组的基因树.

主要成果:

印度尼西亚LSDV分离物被确定为Neethling疫苗菌株和LSDV_NI-2490.的复合物.
隔离物与在东亚和东南亚发现的其他重组LSDV菌株聚集在一起.
该研究展示了用于LSDV全基因组分析和分离集群的替代,准确的方法.

结论:

印度尼西亚的LSDV分离物是一种复合物,其起源与疫苗和野外菌株有关.
这一发现支持亚洲存在特定的重组血统,与俄罗斯和南亚的不同.
该研究提供了有价值的数据,以了解LSDV的起源和传播,并为有效的控制措施提供信息.

关键词:

在LSDVV中使用.遗传遗传网络是一个遗传遗传网络.再组合剂的使用情况.在SNP中,SNP是SNP.整个基因组的测序.

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