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Maryam Ghazvinian^1,2, Saba Asgharzadeh Marghmalek^1,2, Mehrdad Gholami²

¹Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

BMC infectious diseases

|March 4, 2024

概括

抗生素耐药的Enterococcus菌株显示出有关耐药性模式和普遍存在的病毒性基因,如esp,ace和efaA. 正常植物和环境菌株的高耐药性构成了全球健康的重大挑战.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
抗微生物耐药性抗微生物耐药性

背景情况:

抗生素耐药的Enterococcus菌株代表着一个关键的全球健康挑战.
了解它们对抗生素的敏感性,生物膜的形成和毒性是必不可少的.

研究的目的:

为了研究Enterococcus分离物的抗生素敏感性模式.
为了确定这些分离物的生物膜形成能力.
为了确定特定毒性基因 (esp,ace,efaA) 在Enterococcus中的流行率.

主要方法:

从医院环境和个人收集环境和便样本.
使用形态,生化测试和PCR识别分离物.
通过磁盘亚格扩散进行抗菌敏感性测试.
使用定量微板测定评估生物膜生产.
使用PCR检测毒性基因 (esp,ace,efaA) 的检测.

主要成果:

84个分离物被确定为E. faecalis,61个分离物被确定为E. faecium.
对卡纳米辛 (82.1%在E. faecalis) 和普里斯-达尔夫普里斯 (85.7%在E. faecalis) 观察到高耐药率.
在E. faecalis中,ESP基因是最主要的毒性基因 (73.80%),其次是efaA (60.71%) 和ace (30.95%).
超过68%的菌株是生物膜生产者,在强大的生物膜生产者中经常发现sp和efaA.

结论:

肠球菌菌株表现出明显的毒性基因模式.
在E. faecalis中,esp,ace和efaA基因比E. faecium更为普遍.
在正常植物和环境中的Enterococcus菌株中,高水平的抗生素耐药性是一个重大问题.

关键词:

艾斯艾斯艾斯艾斯在西班牙,西班牙.已经过去了 efaA 抗生素耐药性抗生素耐药性生物膜的形成过程.肠球菌 (Enterococci) 是一种内肠球菌.

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