相关实验视频

Updated: Jul 1, 2025

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

口腔粘膜变化紧密结合蛋白在患有口腔平坦的患者

Xiaomeng Ren¹, Kaiyi Li^1,2, Xin Fang^1,3

¹Department of Oral Medicine, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China.

|March 4, 2024

概括

紧密结合屏障蛋白的表达减少,如奥克卢丁,与口腔平坦的严重程度有关. 这一发现表明这些蛋白质在口腔平坦菌的发展中起着作用.

科学领域:

口腔病理学口腔病理学
分子生物学分子生物学
免疫组织化学免疫组织化学

背景情况:

口腔平坦 (OLP) 是一种慢性炎症状况,影响口腔粘膜.
紧接点 (TJs) 在OLP病变发生过程中的作用尚不清楚.
研究TJ蛋白表达可能会揭示OLP发展的新见解.

研究的目的:

检查OLP组织中关键紧结蛋白的表达和分布.
确定紧结蛋白表达与OLP临床严重程度之间的关联.
探索TJs在OLP背后的分子机制中的潜在作用.

主要方法:

从OLP患者和健康对照的口腔粘膜组织的转录组测序.
免疫组织化学和定量逆转录PCR验证TJ蛋白表达 (克劳丁-1,克劳丁-4,奥克卢丁,Zonula occludens-1).
相关性分析将TJ蛋白水平与OLP严重程度联系起来.

主要成果:

在OLP组织中观察到紧密结基因 (CLDN1,CLDN4,OCLN,TJP1) 的显著下调.
克劳丁-1,克劳丁-4,奥克卢丁, zonula occludens-1 蛋白质表达在OLP中显著降低.
减少奥克卢丁表达与OLP严重程度有正相关性.

结论:

紧密结合屏障蛋白的表达减少可能有助于口腔平坦的发展.
奥克卢丁的下调与口腔平坦的严重程度增加有关.
针对紧密结合完整性可能是OLP的潜在治疗策略.

关键词:

在口腔上皮质屏障.在口腔中,有平坦的海.紧密的交叉路口紧密的连接转录组分析转录组分析

更多相关视频

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Noninvasive Sampling of Mucosal Lining Fluid for the Quantification of In Vivo Upper Airway Immune-mediator Levels

Published on: August 7, 2017

Author Spotlight: Oral Candida Diagnosis to Advance Clinical Treatment Regimen for pSS Patients

Author Spotlight: Oral Candida Diagnosis to Advance Clinical Treatment Regimen for pSS Patients

Published on: March 1, 2024

相关概念视频

Tight Junctions

Tight Junctions

Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...

Adherens Junctions

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic