毒素类素与分泌蛋白相互作用,对寄生虫的增殖至关重要
Chenghuan Li1,2, Ning Jiang1,2, Yize Liu1,2
1Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang, 110866, China.
毒素原虫的蛋白质sortilin是一种蛋白质.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 药物发现 药物发现 药物发现
背景情况:
- 人类索尔提林是癌症研究的关键目标.
- 托克索普拉斯马淋菌的sortilin促进蛋白质运输到寄生虫的顶端细胞器.
- 了解T. gondii sortilin对于开发新的抗寄生虫治疗方法至关重要.
研究的目的:
- 确定T. gondii sortilin. 的细胞内定位和结构域.
- 探索用于新型T. gondii治疗的sortilin的功能抑制.
主要方法:
- 在T. gondii. 中基因鉴定和免疫沉.
- 质谱测量用于识别运输的蛋白质.
- 生物层干涉测量以分析域相互作用和抑制剂结合.
主要成果:
- 确定了Vps10,sortilin-C和sortilin-M作为蛋白质运输的结合区域.
- AF38469 抑制剂与 Vps10 域结合,从而抑制 T. gondii.
- AF38469在小鼠T. gondii感染模型中表现出治疗效果.
结论:
- T. gondii sortilin的Vps10,sortilin-C和sortilin-M子域对于蛋白质运输至关重要.
- Vps10子域是抑制剂AF38469.9的结合部位.
- 索尔提林是治疗T. gondii感染的有希望的药物标.
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