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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Jul 1, 2025

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

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显微镜中的高强度自动聚焦方法使用基于激光的阵列斑点.

Zhihao Wang, Xin Zhang, Xindong Chen

    Optics express
    |March 5, 2024
    PubMed
    概括
    此摘要是机器生成的。

    一种新的基于激光的自动对焦方法使用排列的斑点来提高显微镜的精度. 这提高了像Stereo-seq这样的应用程序的系统稳定性,克服了传统方法的局限性.

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    Last Updated: Jul 1, 2025

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    科学领域:

    • 显微镜学和光学工程
    • 先进的成像技术,以及先进的成像技术.

    背景情况:

    • 目前的光电自动对焦方法受到单点焦平面检测的限制.
    • 这种限制在Stereo-seq等应用中降低了系统的稳定性,特别是在不均的样本表面.
    • 边缘检测和样本不一致性对现有的自动对焦系统构成挑战.

    研究的目的:

    • 为了提高显微镜中的自动对焦系统的稳定性和准确性.
    • 为了克服传统光电自动对焦方法的局限性.
    • 引入一种基于激光的新阵列斑点方法,用于主动自动对焦检测.

    主要方法:

    • 提出了一种基于激光的阵列斑点光电自动对焦方法.
    • 包含一个2D-Dammann格子,用于均的光分裂.
    • 在样本表面生成一个n × n的点数组进行检测.

    主要成果:

    • 实现了±100μm的自动对焦范围.
    • 显示了±1/4的自动对焦准确度. 场深度 (DOF).
    • 经过验证的性能与10×目标镜头,满足微观聚焦要求.

    结论:

    • 基于激光的阵列光自动对焦方法为主动自动对焦检测提供了一种新的方法.
    • 这种方法显著提高了自动对焦系统的稳定性和准确性.
    • 该技术在需要精确显微镜聚焦的领域具有相当大的应用价值.