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从室内空气微生物群样本中检测致病细菌的方法,使用高通量安普利康序列测序
Nor Husna Mat-Hussin1, Shing Wei Siew1, Mohd Norhafsam Maghpor2
1Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Persiaran Tun Khalil Yaakob, Gambang, Kuantan, Pahang 26300, Malaysia.
MethodsX
|March 5, 2024
概括
医院的室内空气是有害微生物的息地. 独立于培养的安普利康序列测定提供了一种全面的方法来识别空气中的细菌,改善对乘客的健康风险评估.
科学领域:
- 环境微生物学环境微生物学
- 职业健康 职业健康 职业健康 职业健康
- 传染病控制 传染病控制
背景情况:
- 室内空气,特别是医院的空气,可以容纳对居住者健康有害的致病微生物.
- 由于无法培养或生长缓慢的细菌,传统的依赖培养的方法不足以表征复杂的空气微生物群.
研究的目的:
- 评估一种独立于培养的方法,用于在医院环境中进行全面的空气微生物组分析.
- 为监测有害空气细菌和减轻健康风险提供更有效的工具.
主要方法:
- 使用了针对16S rRNA的V3区域的amplicon测序.
- 分析了从医院内部收集的尘埃样本中提取的全基因组DNA.
- 采用文化独立的方法来绕过传统种植技术的局限性.
主要成果:
- 独立于培养的方法提供快速,全面和公正的空气传播细菌概况,包括致病和非致病物种.
- 安普利康测序使细菌识别能够达到最低的分类学水平,超过了依赖培养的方法的分辨率.
结论:
- 与传统技术相比,安普利康测序是描述医院室内空气微生物群的优越方法.
- 这种方法有助于职业卫生从业者,研究人员和当局监测和控制空气中的微生物风险.
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