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生物信息学分析确定了TGF-β信号通路相关的分子亚型和糖尿病脚中的基因特征

Guanggang Du^1,2, Jie Chen^1,2, Xuezhu Zhu^1,2

¹Department of Burn and Wound Repair, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China.

|March 5, 2024

概括

转化生长因子β (TGF-β) 信号在糖尿病足 (DFU) 中是活跃的. 像TGFBR1和TGFB1这样的关键基因被确定为DFU诊断和理解免疫细胞透的关键.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
生物信息学是一种生物信息学.

背景情况:

转化生长因子β (TGF-β) 在炎症和免疫系统中起着已知的作用.
在糖尿病足 (DFU) 中,TGF-β信号通路相关基因 (TRGs) 的特定机制尚不清楚.

研究的目的:

调查TRG在DFU中识别,分类和理解免疫透中的作用.
确定与DFU发展和进展相关的关键TRG.

主要方法:

在DFU对基因表达数据的生物信息学分析.
差异基因表达分析以确定上调的TRG.
定量实时PCR用于核基因的验证.
使用核心基因开发诊断模型.

主要成果:

在DFU中,TGF-β信号通路被激活,33个TRG被上调.
确定TGFBR1和TGFB1是显著的差异性表达核心基因.
结合这些核心基因的诊断模型显示出高临床有效性 (AUC = 0.909).
核心基因表达与DFU中的免疫细胞透模式相关.
观察到与TGF模式相关的5672个基因的显著差异表达,影响生物功能和免疫透.

结论:

TGF-β信号通路在DFU的发展中起着至关重要的作用.
特定的TRG,如TGFBR1和TGFB1,是DFU的潜在诊断生物标志物.
了解TRG表达模式可以为DFU内的免疫反应提供见解.

关键词:

卫生科学卫生科学医学医学医学医学医学自然科学自然科学

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