使用光学 tweezers 的完全角分辨率的 3D 微观学
Andrew B Matheson1, Tania Mendonca2, Matthew G Smith3
1School of Engineering and Physical Sciences, Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot Watt University, Edinburgh, UK.
Rheologica acta
|March 5, 2024
概括
使用光学 tweezers (MOT) 的微整形学现在可以准确地测量任何方向的材料特性. 一种新的方法纠正了光学陷异构性,大大减少了软材料粘度测量的错误.
科学领域:
- 软物质物理学 软物质物理学
- 材料科学 材料科学 材料科学
- 生物物理学的生物物理.
背景情况:
- 用光学 tweezers (MOT) 探测微观尺度上的粘弹性特性,对于生物样本等复杂材料来说是理想的.
- 3D MOT与粒子跟踪相结合,可以绘制材料性质的空间和方向变化.
- 光学陷固有的3D异形性可以导致在某些方向上显著高估流体粘度.
研究的目的:
- 开发和验证一种新的分析方法,以克服3DMOT中光学陷异性质的工件.
- 为了能够准确地测量任意方向的质性质,克服以前的局限性.
主要方法:
- 主要组件分析 (PCA) 应用于3D MOT数据以表征陷异构性.
- 确定陷异型性影响测量的频率范围.
- 使用模拟的牛顿流体数据和对水和凝溶液的实验测量进行验证.
主要成果:
- 新的分析方法在模拟的牛顿流体数据中将最大粘度误差从~150%降低到<6%.
- 在水和凝溶液上的实验MOT测量证实了可靠的微风学提取.
- 该方法允许在广泛的频率范围和任何方向上准确地确定质性质.
结论:
- 一种新的分析方法有效地纠正了3DMOT中的光学陷异性.
- 这种方法可以对软材料进行准确的,独立于方向的微观学测量.
- 这项工作有助于完全空间和角度分辨率的3D绘制软材料的湿原性质.
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