长期储存导致的血液学参数变化,用于测试
Elise Schapkaitz1, Dashini Pillay2
1Department of Molecular Medicine and Haematology, Charlotte Maxeke Johannesburg Academic Hospital, National Health Laboratory System Complex and University of Witwatersrand, South Africa.
African journal of laboratory medicine
|March 5, 2024
概括
在4°C-8°C保存7天的血液样本,可维持ADVIA® 120分析的全血细胞计 (FBC) 和网状细胞参数. 然而,这种温度不适合差异计数和外周血液涂抹形态检查.
科学领域:
- 血液学 血液学 血液学
- 临床病理学 临床病理学
- 实验室医学 实验室医学
背景情况:
- 来自远程实验室的血液学疾病的延迟分析会影响患者的护理.
- 评估样本稳定性对于准确的远程诊断至关重要.
研究的目的:
- 为了评估全血细胞计数 (FBC),差异计数 (DIFF),网状细胞和周围血液涂抹 (PBS) 形态学的稳定性.
- 为了确定EDTA保存的血液样本的最佳储存条件.
主要方法:
- 在ADVIA® 120分析仪上分析了40个EDTA血液样本 (25%异常).
- 在室温 (RT) 和4°C8°C下存储样品.
- 在各种间隔进行分析,最长为7天的冷藏样品和48小时的RT样品.
主要成果:
- 在RT时,FBC和一些DIFF参数在48小时内保持稳定.
- 血小板和白细胞计数在RT时表现出有限的稳定性 (分别为12小时和36小时).
- 冷却 (4°C8°C) 改善了平均细胞体积和网状细胞的稳定性,但降低了DIFF参数稳定性;PBS形态在12小时内降解.
结论:
- 在4°C8°C保存7天后,EDTA血液样本适合在ADVIA® 120上进行FBC和网状细胞分析.
- 冷藏并不能保持DIFF或PBS的形态.
- 针对不同的血液学参数,需要优化存储协议.
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