在瑞士白鼠模型中,三种Trypanosoma brucei rhodesiense的毒性和致病性稳定
Christopher Kariuki1, John M Kagira2, Victor Mwadime1
1Institute of Primate Research, Kenya.
African journal of laboratory medicine
|March 5, 2024
概括
对Trypanosoma brucei rhodesiense的IPR-001稳定体显示出对小鼠慢性人类非洲三虫病 (HAT) 的建模有希望. 其他测试的稳定物不适合HAT研究的晚期阶段.
科学领域:
- 医学寄生虫学 医学寄生虫学
- 传染性疾病 传染性疾病
- 动物模型 动物模型
背景情况:
- 开发可靠的实验模型对于理解人类非洲试索米亚症 (HAT) 病原和药物疗效至关重要.
- 目前的模型需要改进,以准确模拟疾病.
研究的目的:
- 在瑞士白色小鼠中描述三种Trypanosoma brucei rhodesiense稳定体 (ILRI-2918,ILRI-3953,IPR-001) 的毒性和致病性.
- 为了确定一个合适的稳定物来建模晚期HAT.
主要方法:
- 瑞士白色小鼠被T. b. rhodesiense稳定体感染.
- 寄生虫血症通过湿血涂抹进行监测.
- 晚期疾病诱导和治愈治疗分别使用diminazene aceteturate和melarsoprol进行.
主要成果:
- 专利前的时间从3-4天不等,寄生病的峰值在Log10 6.40-8.36.3之间.
- 存活时间有很大的不同,从6 DPI (ILRI-3953) 到86 DPI (IPR-001).
- ILRI-2918和ILRI-3953呈现出快速进展,不适合晚期建模;IPR-001显示出慢性疾病的潜力.
结论:
- 稳定剂ILRI-2918和ILRI-3953不适合在小鼠中建模晚期HAT.
- 稳定型IPR-001证明了诱导慢性试体病的潜力,使其成为开发晚期HAT模型的候选者.
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