通过不同的捕获方法固定细胞质的表征及其用于胡卜汁提取的应用
Bhaskar Jyoti Kalita1, Nandan Sit1
1Department of Food Engineering and Technology, Tezpur University, Tezpur, Assam 784028 India.
Food science and biotechnology
|March 5, 2024
概括
使用藻酸盐或 agar 固定细胞质可提高其效率和稳定性. 这两种方法都提高了胡卜汁的产量,证明了固定酶的实际应用.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 生物化学 生物化学
背景情况:
- 细胞酶是各种工业应用中的关键酶.
- 酶固定是提高酶稳定性和可重复使用性的关键技术.
- 了解对酶动力学的固定效应对于过程优化至关重要.
研究的目的:
- 使用藻酸盐封装和阿加矩阵捕获来固定细胞质.
- 为了评估固定细胞酶的效率,运动性质和可重复使用性.
- 评估固定细胞酶对胡卜汁产量的影响.
主要方法:
- 通过藻酸盐封装和 agar 捕获来固定细胞质.
- 确定固定效率. 固定效率的确定.
- 在不同的条件下进行酶动力分析 (Km,Vmax).
- 在多个循环中评估酶活性.
- 评估胡卜汁生产中的酶疗效.
主要成果:
- 被困的立方体实现了97.63%的固定效率,高于藻酸盐珠 (92.11%).
- 与自由细胞相比,固定细胞酶表现出改变的最佳条件和动力参数 (Km,Vmax).
- 固定化的细胞质保持了多达5个周期的活性.
- 固定和自由细胞酶都有效地增加了胡卜汁产量.
结论:
- 使用阿加或藻酸盐来固定细胞质是非常高效的.
- 固定改变了酶动力学,但提高了工业应用的稳定性和可重复使用性.
- 固定细胞酶为提高胡卜汁生产效率提供了一种可行的方法.
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