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Updated: Jun 13, 2026

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Published on: November 12, 2014

介绍RSC Advances主题系列关于从大规模模拟中对生物分子系统的新见解

Megan L O'Mara1, Sarah Rauscher2,3,4, Stacey D Wetmore5

  • 1The University of Queensland, Australian Institute for Bioengineering and Nanotechnology Building 75, Cnr College Rd &, Cooper Rd St Lucia Queensland 4067 Australia.

RSC advances
|March 5, 2024
PubMed
概括

本系列使用大规模模拟来探索生物分子系统. 发现对分子行为和功能的新见解.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...