增加Wnt5a/ROR2信号与阴茎退行性阴茎退行性疾病中的冠状体发生有关
Yusuke Inoue1, Ken Kumagai1, Kimi Ishikawa1
1Department of Orthopaedic Surgery and Muscloskeletal Science, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
概括
阴茎退化涉及转向一种冠状体表型的转变,标志着改变的Wnt信号通路. 增加的Wnt5a/ROR2表达和减少的Wnt/β-catenin信号与膝关节骨关节炎中的这种冠状动态发生相关.
科学领域:
- 生物化学 生化学
- 整形外科 整形外科 整形外科
- 细胞生物学 细胞生物学
背景情况:
- 阴茎退化是一个复杂的过程,通常与膝关节关节炎 (OA) 相关.
- 细胞和分子机制驱动半径变性,特别是转向一种体性表型的转变,仍然不完全理解.
- Wnt信号通路与各种发育和疾病过程有关,包括软骨形成和退化.
研究的目的:
- 为了研究在人类阴茎退行期间的体差异化和Wnt信号表达之间的关联.
- 为了比较患有和没有OA的患者的脑膜中Wnt信号元件和chondrogenic标记物.
- 阐明特定Wnt信号通路在变性脑膜变性中观察到的表型变化中的作用.
主要方法:
- 从患有和没有膝关节OA的患者获得了脑膜.
- 使用分级系统对阴茎退化的组织学评估.
- 免疫组织化学,实时PCR和西部斑分析以评估原体标记物 (SOX9,原体II型,原体XI型,aggrecan) 和Wnt信号元件 (Wnt5a,ROR2,TCF7,AXIN2).
主要成果:
- 阴茎变性表现出从纤维细胞转变为冠状体表型的转变,并对冠状体标志物的上调调节.
- 增加Wnt5a和ROR2的表达,以及减少TCF7的表达,在阴茎退行症期间观察到.
- 与非OA阴道相比,OA阴道表现出显著更高的Wnt5a和ROR2表达和显著更低的AXIN2和TCF7表达.
结论:
- 增加Wnt5a/ROR2信号似乎在经阴茎退行期间促进了冠状体生成.
- 下游Wnt/β-catenin信号元件的表达率下降与这种chondrogenic转移有关.
- 这些发现凸显了Wnt信号失调在阴茎退化和OA病变发生过程中的关键作用.
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