基因组甲基转移酶SMYD1是由脂肪组织中的热原刺激诱导的
Annunziata G Cicatiello1, Annarita Nappi1, Fabiana Franchini1
1Department of Clinical Medicine & Surgery, University of Naples 'Federico II', 80131, Naples, Italy.
Epigenomics
|March 5, 2024
概括
基因组甲基转移酶SMYD1在暴露于寒冷和高脂肪饮食期间在脂肪组织中升级. SMYD1通过增加线粒体含量和色脂肪细胞中的Pgc-1α表达来促进能量控制.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 代谢过程中的代谢.
背景情况:
- 脂肪组织在能量恒温中发挥着至关重要的作用.
- 白色脂肪组织 (WAT) 储存能量,而棕色和色脂肪组织通过热生成消散能量.
- 了解脂肪组织功能的调节对于代谢健康至关重要.
研究的目的:
- 在白色和棕色脂肪组织中研究组织甲基转移酶SMYD1的表达模式.
- 检查SMYD1在前脂肪细胞分化成白色和色脂肪细胞中的作用.
- 阐明SMYD1在调节脂肪组织能量消耗中的功能.
主要方法:
- 使用的C57BL/6J小鼠接受了高脂肪饮食和寒冷暴露.
- 使用的3T3-L1细胞分化为白色和色脂肪细胞.
- 评估了SMYD1表达及其对线粒体生物发生和Pgc-1α水平的影响.
主要成果:
- 在高脂肪饮食小鼠的WAT和在暴露于寒冷的小鼠的WAT和棕色脂肪组织中,SMYD1的表达升高.
- 与白色脂肪细胞相比,色脂肪细胞中的SMYD1表达显著更高.
- 沉默SMYD1降低了分化脂肪细胞中的线粒体含量和Pgc-1α表达.
结论:
- SMYD1与脂肪组织对高脂肪饮食和感冒等代谢挑战的适应性反应有关.
- SMYD1作为能量消耗的积极调节者,特别是在色脂肪组织中.
- 这些发现突出了SMYD1在控制脂肪组织内的能量平衡方面的新作用.
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