一个编码beta-lactamase的基因的细胞系间循环:一种罕见但可观察到的事件
Rémi Gschwind1, Marie Petitjean1,2, Claudine Fournier3,4,5
1Université Paris Cité, INSERM, Université Sorbonne Paris Nord, IAME, Paris, France.
Antimicrobial agents and chemotherapy
|March 5, 2024
概括
我们报告了第一个扩展光谱β-乳糖酶 (ESBL) 编码基因在与远距离相关的细菌,Pseudomonadota和Bacteroidota类之间转移的例子. 虽然很少见,但这种跨系基因转移突出显示了抗生素耐药性传播的潜在新途径.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 通过β-乳酸酶介导的耐药性在格拉姆阴性细菌中很常见.
- 基因转移通常发生在密切相关的细菌之间.
- 之前没有记录过β-乳糖酶基因的跨系转移.
研究的目的:
- 报告发现了一种扩展谱β-乳糖酶 (ESBL) 编码基因,该基因共享在Pseudomonadota和Bacteroidota类之间.
- 调查已识别的ESBL基因的基因组背景和进化史.
- 评估这种耐药性基因的流行率和跨系传播潜力.
主要方法:
- 扩展光谱β-乳糖酶的全基因组测序产生大肠杆菌菌株.
- 克隆和β-乳酸酶的生化特征.
- 使用RefSeq基因组和全球微生物基因目录 (GMGC) 进行基因分布的生物信息分析.
主要成果:
- 在大肠杆菌的移动基因组群岛内发现了一个编码ESBL的基因 - - *bla*MUN-1.
- *bla*MUN-1 在 Bacteroidales (Bacteroidota) 和 *Sutterella wadsworthensis* (Pseudomonadota) 两种物种中都被发现.
- 该基因最接近的同类基因经常在人类便样本中检测到,这表明它存在于肠道微生物群中.
结论:
- 这项研究提供了第一个证据,证明了ESBL编码基因在Bacteroidota和Pseudomonadota之间跨系转移.
- 这些发现表明,虽然存在跨系障碍,但它们在防止抗生素耐药性基因传播方面并不是绝对的.
- 在人类肠道中经常检测到这种基因,这表明该基因的储存库,罕见但显著的类间转移事件发生.
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