拥有二基亚酸分流的大肠杆菌利用5'-脱氧核化物进行生长
Katherine A Huening1, Joshua T Groves1, John A Wildenthal1
1Department of Microbiology, The Ohio State University, Columbus, Ohio, USA.
Microbiology spectrum
|March 5, 2024
概括
双基酸盐 (DHAP) 突变允许某些大肠杆菌使用S-adenosyl-l-methionine副产品作为碳来源. 这一途径对于在特定环境中的生长至关重要,特别是对于肠外致病菌大肠杆菌.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 细菌病原体的产生
背景情况:
- 所有生物都在生命过程中使用S-adenosyl-l-methionine (SAM),产生5'-deoxy-nucleoside副产品,如5'-methylthioadenosine (MTA) 和5'-deoxyadenosine (5dAdo).
- 在一些肠外致病菌大肠杆菌中存在二氧酸 (DHAP) 旁路,但其代谢作用尚不清楚.
- 虽然一些细菌使用DHAP分流进行氨酸合成或排毒,但其在大肠杆菌中的功能仍未知.
研究的目的:
- 研究大肠杆菌中DHAP分流通路的功能.
- 确定DHAP分流是否允许利用5'-脱氧核酸副产品作为生长基质.
- 了解DHAP分流对大肠杆菌生长的环境相关性.
主要方法:
- 在大肠杆菌K-12中将DHAP分流通路的遗传引入.
- 在各种条件下 (有氧,无氧与TMAO,发酵,酸盐呼吸) 使用5'-脱氧腺素 (5dAdo) 和MTA作为唯一的碳来源的生长实验.
- 对代谢副产品的分析,包括硫化合物积累.
主要成果:
- 通过DHAP分流,大肠杆菌可以利用5dAdo和MTA作为增长的碳来源.
- 在有氧呼吸和无氧呼吸中观察到与三甲基胺N氧化物 (TMAO) 的增长,但在发酵或酸盐呼吸期间没有.
- 来自MTA的硫不被有效地回收成甲因,而是以2-甲基乙醇的形式积累.
结论:
- DHAP突变为大肠杆菌提供了一个代谢无处不在的SAM利用副产品的机制,将其转化为可用的碳和能源.
- 这一途径在氧化或富含TMAO的环境中对肠外致病性大肠杆菌尤为重要.
- DHAP突变扩大了大肠杆菌可访问的糖化合物的范围,突出了其在特定的适应和生存中的作用.
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