lncRNA JPX通过与皮肤黑色素瘤中的YTHDF2相互作用和破坏稳定,促进瘤的进展
Dan Luo1, Hui Tang1, Liuchang Tan2
1Department of Dermatology, Daping Hospital, Army Medical University, Chongqing, China.
Molecular cancer research : MCR
|March 5, 2024
概括
长非编码RNAJPX通过破坏YTHDF2蛋白质的稳定性,影响细胞增殖和迁移,促进黑色素瘤的进展. 准JPX/USP10/YTHDF2/BMP2通路为黑色素瘤提供了一个潜在的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 长非编码RNAs (lncRNAs) 的异常表达与各种癌症有关.
- 在皮肤黑色素瘤的进展中,lncRNA JPX的特定作用以前尚不清楚.
研究的目的:
- 为了研究 lncRNA JPX 在皮肤黑色素瘤中的功能.
- 阐明JPX在黑色素瘤进展中的作用背后的分子机制.
- 在JPX监管轴中确定潜在的治疗目标.
主要方法:
- 产生了JPX过度表达和JPX抑制的黑色素瘤细胞系 (A375,A2058).
- 在体外测试包括CCK-8,殖民地形成,EDU,Transwell和细胞周期分析.
- 在体内研究中使用了皮下植入的瘤模型.
- 研究分子机制使用RNA下拉,RNA免疫沉降,共免疫沉降,西斑和RNA测序.
主要成果:
- 在黑色素瘤组织和细胞系中,JPX表达显著上调.
- 在体外和体内,JPX knockdown 抑制了黑色素瘤细胞的增殖和迁移.
- JPX与YTHDF2直接相互作用,阻碍USP10介导的保护,并促进YTHDF2的脱和降解.
- 这导致BMP2mRNA稳定和AKT酸化激活.
结论:
- 通过调节YTHDF2无处不在和稳定性,JPX促进皮肤黑色素瘤的进展.
- 这项研究揭示了一种新的JPX/USP10/YTHDF2/BMP2轴涉及黑色素瘤.
- 针对这一轴为皮肤黑色素瘤治疗提供了一个有希望的治疗途径.
相关概念视频
Tumor Progression
6.3K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.3K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
Induced Pluripotent Stem Cells
4.1K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.1K
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Loss of Tumor Suppressor Gene Functions
4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K


