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工程化蛋白酶响应RNA结合蛋白 (RBPs) 以扩大哺乳动物细胞中合成电路的工具箱

Fabiana Calandra¹, Velia Siciliano²

¹Synthetic and Systems Biology lab for Biomedicine, Istituto Italiano di Tecnologia-IIT, Naples, Italy.

Methods in molecular biology (Clifton, N.J.)

|March 5, 2024

概括

研究人员设计了蛋白酶响应的RNA结合蛋白 (RBPs),以增强哺乳动物细胞中的合成基因电路. 这扩大了为生物医学应用创建复杂,多层细胞调节的工具包.

科学领域:

合成生物学合成生物学
分子和细胞生物学分子和细胞生物学
生物技术是生物技术.

背景情况:

基因编码的传感器-执行器电路使用输入输出信号重新编程细胞功能.
转录后基因调节,利用RNA结合蛋白 (RBPs) 等元素,对于先进的生物医学应用至关重要.
现有的合成电路在监管正交度方面遇到了局限性,阻碍了复杂的网络建设.

研究的目的:

为扩大合成生物学工具箱设计新型蛋白酶响应RNA结合蛋白 (RBPs).
提高哺乳动物细胞中转录后基因调节的能力.
允许在合成电路中创建复杂的多层监管网络.

主要方法:

特定的RNA结合蛋白 (L7Ae和MS2-cNOT7) 的工程,以响应蛋白酶活性.
在哺乳动物细胞系统中测试工程RBPs的功能性和响应性.
评估使用这些工程RBP创建直角和多层监管网络的潜力.

主要成果:

成功的工程和测试蛋白酶响应RNA结合蛋白 (L7Ae和MS2-cNOT7).
证明了哺乳动物细胞中合成电路的可用调节部分的扩展.
为建立更复杂和更复杂的基因调节网络奠定了基础.

结论:

蛋白酶响应的RBP提供了一种用于控制基因表达后转录的新机制.
设计的RBP增强了合成基因电路的直角性和分层能力.
这项工作为推进合成生物学在生物医学研究和应用中提供了有价值的工具.

关键词:

哺乳动物合成生物学蛋白质工程是一种蛋白质工程.蛋白RNA调节蛋白RNA调节蛋白质蛋白质相互作用 RNA结合蛋白质是RNA结合的蛋白质.烟草蚀刻病毒的蛋白酶.

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