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流细胞计量哺乳动物细胞中过渡性转染的量化

¹Raytheon BBN Technologies, Cambridge, MA, USA. jakebeal@ieee.org.

Methods in molecular biology (Clifton, N.J.)

|March 5, 2024

概括

定量流细胞计需要对可重复的,生物相关的单细胞数据进行过程控制. 这种方法增强了工程生物系统的开发和分析.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
定量生物学定量生物学

背景情况:

流细胞计是收集单细胞数据的高通量方法.
流细胞计中的可复制性依赖于过程控制和统计分析.
量化数据对于理解潜在的生物过程至关重要.

研究的目的:

描述定量流细胞计的方法.
为了使工程生物生物体的更好的开发,建模和调试.
确保可复制,生物相关的单细胞数据收集.

主要方法:

在实验样本上加入适当的工艺控制.
利用工艺控制来进行单位校准和质量控制.
应用合适的统计方法进行数据分析.

主要成果:

使用工艺控制的定量流细胞计的既定方法.
对哺乳动物细胞转移和潜在的其他细胞类型的证明适应性.
为工程生物系统提供了更强大的数据分析.

结论:

使用过程控制的定量流细胞计确保了数据的可复制性和生物相关性.
这些方法支持工程生物生物体的进步.
描述的技术可以适应各种转染类型和细胞类别.

关键词:

流动细胞计量流动细胞计量光测量光测量计量学计量学计量学单细胞生物学单细胞生物学合成生物学合成生物学

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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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