相关实验视频

Updated: Jul 1, 2025

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells

Published on: September 28, 2011

在哺乳动物细胞中使用合成触控开关检测微RNA

Yuwen Zhao^1,2, Pratima Poudel¹, Shue Wang³

¹Department of Chemistry, Chemical and Biomedical Engineering, Tagliatela College of Engineering, University of New Haven, West Haven, CT, USA.

Methods in molecular biology (Clifton, N.J.)

|March 5, 2024

概括

这项研究引入了一种新型的合成RNA脚开关,用于检测各种人体细胞中的微RNA (miRNA). 这个平台可以精确监测疾病和发育相关的细胞机制.

科学领域:

合成生物学合成生物学
分子诊断学分子诊断学
基于RNA的系统是基于RNA的.

背景情况:

合成生物学利用工程基因电路进行细胞控制.
基于RNA的开关对于下一代治疗和诊断至关重要.
现有的RNA开关缺乏对真核细胞转化控制的强有力的表征,特别是基于miRNA的系统.

研究的目的:

设计和描述一种用于检测微RNAs (miRNAs) 的新型合成脚开关.
为了使在各种细胞系中检测出异源和内源的miRNAs.
为了评估合成RNA开关平台的可编程性和正角性.

主要方法:

一个合成脚开关的设计,针对特定的miRNAs.
在中国仓鼠卵巢 (CHO),HeLa,HEK 293和MDA-MB-231乳腺癌细胞中测试开关.
使用两个不同的脚开关,对miR-155和miR-21进行多重检测.

主要成果:

在多个真核细胞细胞系中成功检测出源和内源的miRNA.
对特定miRNAs (miR-155和miR-21) 的多重检测能力的演示.
评估合成平台的可编程性和直角性.

结论:

开发的合成脚开关是用于在真核细胞中检测miRNA的可行平台.
这项技术有可能推进分子诊断和理解细胞机制.
基于miRNA的开关的进一步表征和应用可能会影响治疗和疾病研究.

关键词:

这是一个微型RNA.合成生物学合成生物学合成的脚掌开关是一个人造的脚掌开关.基于miRNA的开关

更多相关视频

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...