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Updated: Jul 1, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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使用基于RNA的光传感器对活细胞中的S-Adenosyl Methionine动态进行成像

Jared D Moon1,2,3, Kevin Yusko4, Lindsey Nassimos4

  • 1Department of Psychiatry, Columbia University, New York, NY, USA. jared.moon@nyspi.columbia.edu.

Methods in molecular biology (Clifton, N.J.)
|March 5, 2024
PubMed
概括

研究人员开发了一种新的方法来可视化细胞中的S-Adenosyl metionin (SAM) 动态. 这项技术使用化RNA传感器实时跟踪SAM,这对于DNA甲基化和基因调节至关重要.

关键词:
光成像成像的使用方法原性阿帕特马尔是一种原性阿帕特马尔.活细胞成像技术使用.它们是RNA受体.基于RNA的光传感器传感器

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Protein Dynamics in Living Cells01:19

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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