相关实验视频

Updated: Jul 1, 2025

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

作为室温RNA储存纳米储存器的共价有机框架

Satyapriya Nath^1,2, Kiran D Tulsiyan^1,2, Binayak Mohapatra^2,3

¹School of Chemical Sciences, National Institute of Science Education and Research (NISER) Bhubaneswar, Jatni, Khurda, Odisha, 752050, INDIA.

Chemistry (Weinheim an der Bergstrasse, Germany)

|March 5, 2024

概括

新型共价有机框架 (COFs) 允许在室温下储存核糖酸 (RNA) 治疗药物. 这一突破解决了RNA不稳定的挑战,为更容易的存储和运输铺平了道路.

科学领域:

材料科学材料科学材料科学
生物技术是生物技术.
有机化学有机化学

背景情况:

核糖核酸 (RNA) 疗法非常有前途,但在环境温度下高度不稳定,需要冷储存.
目前RNA存储,稳定和运输方面的局限性阻碍了它们的临床应用.
开发用于室温RNA保存的方法是一个至关重要的未满足的需求.

研究的目的:

合成和评估共价有机框架 (COFs) 作为室温RNA存储的新型纳米储存器.
研究COF形态和化学特性在RNA稳定中的作用.
为了确认从COF存储中回收的RNA的完整性和可处理性.

主要方法:

基于的离子COF (TTG_Cl) 的合成,具有纳米管形态和中性COF (TMT-TT) 的比较.
在合成的COF中储存RNA的室温.
密度函数理论 (DFT) 研究以阐明COF-RNA相互作用.
使用RNase. 的酶降解试验.
凝电泳和测序以评估RNA完整性和可处理性.

主要成果:

TTG_ClCOFs展示了有效的纳米储存能力,用于在室温下储存RNA.
DFT研究证实了COF和RNA核基之间的非共价相互作用,使可逆RNA负载成为可能.
储存在COF中的RNA表现出对RNase诱导的酶降解的抗性.
恢复后的RNA保持了结构完整性,并且适合进一步处理,经过凝电泳和测序证实了这一点.

结论:

基于的离子COF,特别是具有纳米管形态的TTG_Cl,为室温RNA存储提供了可行的解决方案.
开发的COF系统保护RNA免受降解,并为下游应用保持其完整性.
这一进步对基于RNA的治疗方法的实际开发和交付有重大影响.

关键词:

共价有机框架是共价有机框架.离子离子是有离子的.纳米管是一种纳米管.储存RNA的RNA是可以储存的.恢复恢复恢复恢复恢复

更多相关视频

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

相关概念视频

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...