高通量毛细体液体染色学使用滴滴注射和应用到反应查
Yue Xin1, Samuel W Foster2, Devin M Makey1
1Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109, United States.
Analytical chemistry
|March 5, 2024
概括
一个新的滴滴注入器系统通过将循环时间缩短到5秒,显著提高了液态染色学 (LC) 的吞吐量. 这项创新使得高速化学反应选能够减少浪费.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
- 质谱测量质量谱测量
背景情况:
- 标准的液态染色学 (LC) 系统面临吞吐量限制,原因是长的自取样器注射序列 (>15秒).
- 传统液压控制系统中的大孔柱产生大量的移动相废物,并对质谱界面构成挑战.
- 由于周期时间的限制,现有的LC方法难以实现高通量选.
研究的目的:
- 开发一个滴滴注入器系统,以克服LC中传统自动采样器的局限性.
- 为了使高通量分析和化学反应选使用毛细血管LC.
- 为了减少LC分离中的移动阶段和样品消耗.
主要方法:
- 设计了一种液滴注入器系统,采用四口,双位置,内环20nL,与注射器和三轴定位器接口.
- 该系统交替从井板抽取样品滴和不混合的液体进入毛细血管.
- 使用毛细体LC柱 (内部直径300微米) 来最大限度地减少溶剂和样品的使用.
主要成果:
- 滴滴注入器系统实现了5秒的循环时间,允许连续注入20nL样品滴.
- 该系统在8.1分钟内成功进行了96次分离3组分样本.
- 通过电子喷射电离与质谱仪的合方便了高通量化学反应选.
结论:
- 开发的液滴注入器系统显著提高了LC的吞吐量,并减少了循环时间.
- 该方法为高通量选提供了可行的解决方案,可减少移动阶段和样品消耗.
- 该系统展示了与质谱学的有效接口,用于先进的分析应用.
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