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聚乙烯纳米颗粒诱导了 Pseudomonas aeruginosa 中的生物膜的形成

Pan Huang¹, Zun Li¹, Ruidan Liu¹

¹Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai International Advanced Research Institute (Shenzhen Futian), Nankai University, Tianjin, China.

Journal of hazardous materials

|March 5, 2024

概括

聚烯纳米颗粒 (PS-NPs) 通过诱导氧化应激和改变膜透性,促进细菌生物膜的形成和Pseudomonas aeruginosa PAO1中的抗生素耐药性. 细菌通过定数感应和增加运动性来增强防御能力.

科学领域:

环境科学环境科学
微生物学微生物学
毒理学毒理学毒理学

背景情况:

微型/纳米塑料带来了重大的生态风险,需要对其生物影响进行研究.
Pseudomonas aeruginosa PAO1 是一个相关的模型生物体,用于研究细菌对环境压力因素的反应.

研究的目的:

研究不同尺寸的聚乙烯纳米颗粒 (PS-NP) 对 Pseudomonas aeruginosa PAO1 的生长和生物膜形成的影响.
阐明PS-NP影响细菌行为和生理学的潜在机制.
评估PS-NP对细菌抗生素耐药性的影响.

主要方法:

暴露 Pseudomonas aeruginosa PAO1 在不同度的聚乙烯纳米颗粒 (PS-NP).
对细菌生长,生物膜形成,膜透性和氧化应激标志物的分析.
生物膜相关基因的基因表达分析和定数感应 (QS) 系统组件.
评估细菌的运动性和细胞外聚合物质 (EPS) 和毒性因子的分泌.

主要成果:

某些度的PS-NP显著促进了P. aeruginosa PAO1.1.中的细菌生物膜的形成.
通过直接接触或透,PS-NPs诱导了氧化应激和改变了细菌膜的透性.
细菌通过QS系统调节了生物膜的形成,EPS和毒性因子的分泌和运动,以抵消PS-NP的毒性.
暴露于PS-NP增强了细菌抗生素耐药性.

结论:

聚乙烯纳米颗粒对细菌产生有毒作用,触发防御机制,包括增强生物膜形成和运动性.
定数感应系统在调解细菌对纳米塑料暴露的反应中起着至关重要的作用.
纳米塑料污染对抗微生物治疗构成挑战,原因是细菌抗生素耐药性增加.
这些发现对环境纳米塑料风险评估和管理策略具有重要意义.

关键词:

抗生素耐药性抗生素耐药性生物膜的形成过程.纳米塑料是一种纳米塑料.有毒性机制有毒性机制

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