微生物社区和抗菌素耐药性利基区分在一个多阶段的,表面流量构建湿地
Franciszek Bydalek1, Gordon Webster2, Ruth Barden3
1Department of Chemical Engineering, University of Bath, Claverton Down, Bath BA2 7AY, UK; Water Innovation and Research Centre (WIRC), University of Bath, Bath BA2 7AY, UK; GW4 NERC CDT in Freshwater Biosciences and Sustainability, Cardiff University, Cardiff CF10 3AX, UK; Organisms and Environment Division, School of Biosciences, Microbiomes, Microbes and Informatics Group, Cardiff University, Cardiff CF10 3AX, UK.
Water research
|March 5, 2024
概括
自由生活的细菌,而不是与粒子相关的细菌,是抗微生物耐药性基因和病原体离开建造湿地的主要途径. 这凸显了管理流动细菌的必要性,以有效处理废水.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 细菌浮游生物以自由生活 (FL) 和粒子相关 (PA) 社区的形式存在,具有不同的移动性.
- 了解它们的独特动态对于优化废水处理至关重要,特别是在消除病原体和抗菌素耐药性 (AMR) 方面.
- 建筑湿地 (CWs) 越来越多地用于废水抛光.
研究的目的:
- 调查PA和FL细菌浮游生物群落和相关抗菌素耐药性基因 (ARG) 的时空动态,在一个全规模的市政废水CW中.
- 为了确定每个分量的对ARG去除和废水负载的贡献.
- 为了确定特定微生物种群,ARG和细菌分数之间的相关性.
主要方法:
- 使用16S rRNA基因测序,对PA,FL和沉积物部分进行社区成分分析.
- 使用qPCR对特定的ARG (tetW, ermB, sul1, intI1) 的量化.
- 研究微生物群落和ARG动态在一个自由水表面抛光CW.
主要成果:
- 帕社区将大多数 (70%) 的抗药性负载引入了CW.
- 尽管有效地固定了PA,但移动FL部分负责出口超过60%的废水AMR负载.
- 在FL细菌,特定的ARG (tetW, ermB) 和潜在的致病类型 (例如,Bacteroides,Enterobacteriaceae) 之间发现了强烈的相关性.
结论:
- 在CW中,微生物群落和相关的AMR存在明显的利基差异化.
- 自由生活的细菌代表了一条重要的,经常被忽视的,从CW传播致病性和ARG传播的途径,特别是在低流量条件下.
- 废水处理策略必须考虑FL细菌的流动性,以改善病原体和AMR控制.
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