转录基因和功能分析显示,布拉西诺固醇无敏1受体 (OsBRI1) 调节了大米的寒冷耐受性
Yi-Shan Cheng1, Ye-Dong Sun1, Jia-Ying Xing1
1Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, 110161, China.
Plant physiology and biochemistry : PPB
|March 5, 2024
概括
类固醇 (BR) 受体OsBRI1对于大米耐寒性至关重要. 失去OsBRI1功能会降低耐寒性,改变生理反应和基因表达,突出其在植物寒冷应激适应中的作用.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 压力生理学 压力生理学
背景情况:
- 铜类固醇 (BR) 是重要的植物激素,调节发育和应激反应.
- 外源的BR应用增强了大米的寒冷耐受性,但潜在的机制尚不清楚.
- 了解寒冷压力下的BR信号对于提高大米弹性至关重要.
研究的目的:
- 为了研究BR受体OsBRI1在米寒冷耐受性中的功能.
- 阐明OsBRI1影响寒冷应激反应的分子机制.
- 为了确定参与大米中BR介导的寒冷耐受性的关键基因.
主要方法:
- 在寒冷压力下对一个OSBRI1功能丧失突变体 (d61-1) 的表征.
- 生理分析测量抗氧化酶活性,蛋白质,糖,MDA和电导率.
- RNA测序 (RNA-seq) 和权重基因共同表达网络分析 (WGCNA) 以确定差异表达基因 (DEG).
- 定量实时PCR (qRT-PCR) 用于基因表达的验证.
主要成果:
- 与野生型 (WT) 米相比,d61-1突变呈现出显著降低的寒冷耐受性.
- d61-1显示保护生理参数 (CAT,SOD,POD,PRO,可溶蛋白,可溶糖) 的水平降低,压力指标 (MDA,REC) 的增加.
- RNA-seq在寒冷压力下揭示了WT和d61-1中的众多DEG,具有明显的表达模式,包括与寒冷压力和激素信号相关的基因.
结论:
- OsBRI1在调解大米耐寒性方面发挥着至关重要的作用.
- 失去OsBRI1功能会损害植物应对寒冷压力的能力,因为它会影响生理防御和基因表达.
- 已识别的基因,包括BRI1关联受体激酶1和MYB转录因子,是通过BR信号通路增强大米耐寒性的潜在目标.
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