菌减轻了鱼的肠道损伤,这是微塑料造成的
Yao Zheng1, Lu Xiaoxian2, Jiawen Hu3
1Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), Wuxi, Jiangsu, 214081, China; Wuxi Fishery College, Nanjing Agricultural University, Wuxi, Jiangsu, 214081, China.
Chemosphere
|March 5, 2024
概括
聚乙烯微塑料 (MPs) 影响着鱼的肠道健康,改变了微生物群落和生化标志物. 菌可缓解一些MP诱导的肠道损伤,为鱼类的微塑料污染提供潜在的保护作用.
科学领域:
- 环境毒理学环境毒理学
- 水生生物学 水生生物学
- 鱼类生理学 鱼类生理学
背景情况:
- 聚乙烯微塑料 (MPs) 对水生生物构成环境风险.
- 在MP表面上的微生物可以产生毒理作用.
- 鱼对MP的反应可能因颗粒大小而异.
研究的目的:
- 研究不同尺寸的聚乙烯微塑料对幼鱼 (Oreochromis niloticus) 的影响.
- 分析MP和Chlorella vulgaris共同暴露对鱼肠健康的影响,使用综合的"omics"方法.
- 评估暴露于MPs的鱼体内的组织病理学变化,酶活动和分子反应.
主要方法:
- 年轻的鱼被暴露在各种大小的MPs (75nm,7.5μm,750μm) 和Chlorella vulgaris中,分别为10天和14天.
- 分析了肠道组织病理,酶活性 (例如,EROD,ATP,LPL,caspase 3),以及生物化学标记物 (TC,TNFα).
- 采用了集成的"omics"工作流程,包括转录组,蛋白组,微生物群和代谢组.
主要成果:
- 在杯状细胞上积累了MPs; 氏菌组显示状细胞融合没有显著的肠道损伤.
- 暴露于MPs改变了肠道总胆固醇 (TC) 和瘤坏死因子α (TNFα) 的水平.
- 蛋白质组学揭示了PPAR信号通路的显著丰富,与actin细胞骨和焦点粘附通路的改变.
- 微生物群分析显示,随着MP暴露,特定细菌种群 (Candidatus latescibacteria,C. uhrbacteria,C. abyssubacteria,C. cryosericota) 的丰度下降.
- 代谢分析表明,长期暴露于MP时,碳酸,醇和脂肪酸的含量增加.
结论:
- 微塑料的大小影响了长期的组织病理学变化,基因/蛋白质表达和肠道微生物代谢物.
- 菌可以缓解MP诱导的肠道组织病理损伤.
- 综合的"omics"方法为MP,鱼肠健康和潜在的缓解策略之间的复杂相互作用提供了全面的见解.
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