从外部质量保证计划中吸取的经验教训,适用于CLSI解释标准,用于报告piperacillin/tazobactam敏感性
Balaji Veeraraghavan1, Yamuna Devi Bakthavatchalam1, Rani Diana Sahni1
1Department of Clinical Microbiology, Christian Medical College, Vellore, India.
Indian journal of medical microbiology
|March 5, 2024
概括
皮佩拉西林/塔扎巴克坦 (PTZ) 的自动化敏感性测试显示MIC值准确,但结果经常被误解. 临床实验室需要更新关于根据CLSI指南解释PTZ MIC的教育.
科学领域:
- 临床微生物学 临床微生物学
- 抗微生物耐药性 抗微生物耐药性
- 药理学 药理学是指药理学的学科.
背景情况:
- 准确的抗微生物敏感性测试 (AST) 对于指导患者治疗至关重要.
- 皮佩拉西林/塔扎巴克坦 (PTZ) 是一种关键的抗生素,其准确的敏感性解释至关重要.
- 临床和实验室标准研究所 (CLSI) 定期更新解释标准.
研究的目的:
- 评估自动化AST的性能,以检测皮佩拉西林/塔扎巴克坦 (PTZ) 最低抑制度 (MIC).
- 评估MIC确定和解释的准确性,并将其与参考微型乳头稀释方法进行比较.
- 根据2022年CLSI指南,突出 PTZ MIC 解释中的差异.
主要方法:
- 通过使用参考微稀释方法 (10次复制) 确定PTZ与Klebsiella pneumoniae临床分离物的模式MIC.
- 通过自动化测试与参考方法获得的MIC值进行比较.
- 分析了434个实验室对2022年CLSI标准的MIC结果的解释.
主要成果:
- 参考方法为PTZ产生了16 mg/L的模态MIC,表明敏感剂量依赖性 (SDD) 活性.
- 虽然434个实验室正确确定了16 mg/L的MIC,但只有301个实验室准确地将其解释为根据2022年CLSI指南的SDD.
- 许多实验室未能根据修订的标准正确解释PTZ MIC结果.
结论:
- 自动化的AST系统可以准确地确定PTZ MICs.
- 临床实验室需要加强对AST结果的解释方面的教育和培训.
- 遵守最新的CLSI指南对AST方法验证和解释的遵守对于有效的抗菌药物管理至关重要.
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