在spliceosome组装过程中,SNU66在维持5'拼接部位身份方面发挥了作用
Kenna Sarka1, Sol Katzman2, Alan M Zahler3
1Center for Molecular Biology of RNA and Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California 95064, USA.
RNA (New York, N.Y.)
|March 5, 2024
概括
在SNRP-27和SNU-66蛋白中的突变破坏了结合体组合,导致C. elegans的替代5'结合部位使用和致命性. 这些发现凸显了SNU-66在维持5'拼接部位身份方面的作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 在RNA生物学,RNA生物学.
背景情况:
- 拼接体,一个复杂的分子机器,负责RNA拼接.
- 在结合体组装过程中,U1 snRNA最初识别了5'结合部位,但随着结合体成熟,其他因素保持了这种识别.
- 结构数据表明,人类SNRP27和SNU66之间的相互作用稳定了关键的结合体结构.
研究的目的:
- 为了研究SNRP-27 M141和SNU-66 H765之间的相互作用在C. elegans. spliceosome组装过程中维持5'拼接部位身份的作用.
- 为了确定这些蛋白质的突变是否会影响替代的5'拼接部位使用.
主要方法:
- 在C. elegansSNRP-27 (M141T) 和SNU-66 (H765G) 蛋白质中产生和分析突变.
- 在突变菌株中评估了替代5'拼接部位的使用.
- 创建了双重突变,并测试了U1 snRNA突变的影响,并交换了5'拼接位.
主要成果:
- 在SNU-66 H765中发生的突变促进了替代的5'拼接部位使用,与SNRP-27 M141突变有显著的重叠.
- 双重突变的snrp-27 (M141T) 和snu-66 (H765G) 是同卵性致命的.
- 这些突变对替代拼接的影响取决于附近较弱的拼接位和潜在的拼接因子结合位.
结论:
- 在SNRP-27 M141和SNU-66 H765之间的相互作用对于在spliceosome组装过程中保持5'拼接位的同一性至关重要.
- 这种相互作用的破坏导致了替代的5'拼接部位使用和致命性.
- 这项研究揭示了SNU-66的C端在调节结合部位选择中的新作用.
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