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相关概念视频

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.8K
Protein Modifications in the RER01:26

Protein Modifications in the RER

5.2K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.2K
Phosphorylation01:02

Phosphorylation

50.3K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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相关实验视频

Updated: Jul 1, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

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喷雾型修改:在翻译后修改中,一个新兴的范式.

Yun-Bin Lee1, Hyun-Woo Rhee2

  • 1Department of Chemistry, Seoul National University, Seoul 08826, Korea.

Trends in biochemical sciences
|March 5, 2024
PubMed
概括

翻译后修改 (PTM) 可以发生非特异性,称为喷雾型PTM. 这挑战了传统的特定序列观点,提出了一个基于酶活性位点基质可用性的机制.

关键词:
通过ADP-Rylation进行ADP化.乙化 乙化是一种乱交 乱交 是一种乱交.靠近的距离 靠近的距离 靠近的距离接近性标签的标签.无处不在的化

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

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相关实验视频

Last Updated: Jul 1, 2025

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
08:12

Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

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科学领域:

  • 生物化学 生化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 翻译后修改 (PTMs) 对蛋白质功能至关重要.
  • 传统上,PTM被认为是特定序列的事件.
  • 新出现的证据表明,非序列特异的PTMs发生在细胞区内.

研究的目的:

  • 为了收集喷雾型PTM的证据.
  • 为非序列特定的PTMs提出一个机制.
  • 为了解PTM引入一个新的概念框架.

主要方法:

  • 文献综述和现有研究的综合.
  • 对PTM机制的分析.
  • 对PTM作家活动的概念建模.

主要成果:

  • 累积的证据支持喷雾型PTM的存在.
  • 一个拟议的机制涉及在PTM编写器活性位点暴露电友基质.
  • 喷雾类型的PTM解释了杂乱的作家事件.

结论:

  • 传统的特定序列模型不足以解释所有PTM.
  • 一个喷雾型PTM框架为PTM监管提供了更广泛的理解.
  • 这一框架有助于理解 PTM 事件,而不是通过序列特异性来解释.