有机光体具有大杆移位,用于快速蛋白质和核酸检测的可视化
Jingkai Yang1, Ziyi Xu1, Le Yu2
1Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, 1088 Xueyuan Road, Shenzhen, 518055, China.
Angewandte Chemie (International ed. in English)
|March 5, 2024
概括
新的光染料为快速检测疾病提供了更高的灵敏度. 这些制成纳米颗粒的小分子光体使得在侧向流量测试中能够用肉眼检测SARS-CoV-2和Mopox核酸.
科学领域:
- 有机化学 有机化学
- 材料科学是一种材料科学.
- 生物医学工程 生物医学工程
背景情况:
- 有机小分子光体由于其可调节性质,对生物传感有很大的希望.
- 经典的光体通常具有光谱重叠,需要复杂的光学设置.
- 这限制了它们在简单的诊断设置中的使用.
研究的目的:
- 开发可调节波长的小分子光染料,具有较大的斯托克斯移位.
- 为了将这些染料制成纳米粒子,用于增强生物传感应用.
- 评估它们在用于疾病检测的横向流量试验中的性能.
主要方法:
- 新型可调节波长的小分子光染料 (PT) 的合成.
- 将 PT 制成光纳米粒子 (PT-NP) 的方法.
- 在SARS-CoV-2和Mopox检测的侧向流量试验 (LFA) 中应用PT-NP,有或没有循环介导同热放大 (LAMP).
主要成果:
- 开发的PT染料与斯托克斯变化高达262nm,高摩尔灭绝系数 (30,000-100,000M-1cm-1),和量子收益率高达54.8%.
- 通过裸眼LFA,PT-NPs通过裸眼LFA实现了SARS-CoV-2核体蛋白的20 fM检测极限,比黄金纳米颗粒提高了100倍.
- 通过LFA-LAMP系统可可视化单复制核酸检测麻疹 (Mpox) 的情况.
结论:
- 新型小分子光染料和纳米粒子为简单而敏感的生物传感提供了显著的优势.
- 这些PT-NP表明了快速的潜力,护理点诊断,提高了灵敏度.
- 开发的LFA系统在低度检测病毒核酸方面表现有前途.
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